Gene Card 基因详情

支持统一新 ID、NCBI Gene ID、GeneID、locus_tag、Transcript ID、Protein ID，以及已人工核对的文献基因名查询。页面会先解析到丹参统一基因，再整合坐标、序列、注释、亚细胞定位、EMS 突变和下载入口。

当前解析结果

`SmilChr05G000345`

染色体: Chr05 坐标: 2558691-2578315 链方向: - 类型: protein_coding

基本信息

统一新 Gene ID	`SmilChr05G000345`
名称	SmilChr05G000345
描述	protein VACUOLELESS1
染色体 / SeqID	Chr05 / NC_080391.1
坐标与链方向	2558691 - 2578315 / `-`

ID 对照

NCBI Gene ID	`LOC130985064`
GeneID	`130985064`
locus_tag	`-`
NCBI Name	`LOC130985064`
原始查询	`SmilChr05G000345`

查看完整 ID 对照表

功能注释

来源	GFF basic annotation; NCBI GO GAF; Pfam-A/HMMER; Arabidopsis/Rice homolog
描述	protein VACUOLELESS1
产物	protein VACUOLELESS1
Arabidopsis	`AT2G38020` (VCL1) vacuoleless1 (VCL1) [Source:NCBI gene (formerly Entrezgene);Acc:818380] identity=70.9; qcov=1.02; evalue=0; confidence=high
Rice	`Os01g0666800` - identity=59.9; qcov=1.04; evalue=0; confidence=high
GO	GO:0003779 (actin binding); GO:0005737 (cytoplasm); GO:0005765 (lysosomal membrane); GO:0005768 (endosome); GO:0006886 (intracellular protein transport); GO:0016197 (endosomal transport); GO:0030897 (HOPS complex); GO:0042144 (vacuole fusion, non-autophagic); GO:0043531 (ADP binding); GO:0098542 (defense response to other organism)
KEGG	-
Pfam	PF00931 (NB-ARC); PF04840 (Vps16_C); PF04841 (Vps16_N); PF13401 (AAA_22); PF23559 (WHD_DRP)
InterPro	-
eggNOG	-
NR	GeneID:130985064

打开注释模块

亚细胞定位 Top 5

Rank	定位位置	中文	Score	Protein ID
1	Cell membrane	细胞膜	0.60000	`XP_057763815.1`
2	Golgi apparatus	高尔基体	0.60000	`XP_057763819.1`
3	Cytoplasmic vesicle	细胞质囊泡	0.60000	`XP_057763821.1`
4	Cytosol	细胞质基质	0.40000	`XP_057763815.1`
5	Clathrin-coated vesicle	Clathrin-coated vesicle	0.40000	`XP_057763819.1`

进入亚细胞定位查询

EMS 诱变突变

下表显示该基因在丹参 EMS 材料中的前 12 个候选突变；完整结果可进入 EMS 突变库继续筛选或下载。

位置	REF	ALT	类型	影响	携带材料数	材料
`Chr5:2563284`	`C`	`T`	EMS_canonical_SNV	CDS_variant	2	B40,C43
`Chr5:2563328`	`C`	`CTT`	2bp_insertion	stop_gained,frameshift_variant	1	B53
`Chr5:2563328`	`C`	`CTT`	2bp_insertion	CDS_variant	1	B53
`Chr5:2563393`	`C`	`T`	EMS_canonical_SNV	CDS_variant	3	B73,B86,C22
`Chr5:2563395`	`C`	`CAA`	2bp_insertion	frameshift_variant	1	C39
`Chr5:2563395`	`C`	`CAA`	2bp_insertion	CDS_variant	1	C39
`Chr5:2563405`	`G`	`A`	EMS_canonical_SNV	CDS_variant	1	C39
`Chr5:2563409`	`CAA`	`C`	2bp_deletion	frameshift_variant	2	B73,B86
`Chr5:2563409`	`CAA`	`C`	2bp_deletion	CDS_variant	2	B73,B86
`Chr5:2563419`	`C`	`T`	EMS_canonical_SNV	CDS_variant	3	A23,A3,B53
`Chr5:2563428`	`C`	`T`	EMS_canonical_SNV	CDS_variant	3	A23,A3,B53
`Chr5:2563430`	`C`	`T`	EMS_canonical_SNV	CDS_variant	5	A19,B26,B58,C21,C31

进入 EMS 突变库

预测功能结构域

结构域结果来自 Pfam-A HMM 数据库和 HMMER hmmscan；位置为对应蛋白序列上的氨基酸坐标。

XP_057763815.1

880 aa

440

880

NB-ARC

WHD_DRP

PF00931 NB-ARC 201-353 aa PF23559 WHD_DRP 438-507 aa

XP_057763816.1

880 aa

440

880

NB-ARC

WHD_DRP

PF00931 NB-ARC 201-353 aa PF23559 WHD_DRP 438-507 aa

XP_057763817.1

841 aa

420

841

NB-ARC

AAA_22

WHD_DRP

PF00931 NB-ARC 154-317 aa PF13401 AAA_22 168-254 aa PF23559 WHD_DRP 401-468 aa

XP_057763818.1

841 aa

420

841

NB-ARC

AAA_22

WHD_DRP

PF00931 NB-ARC 154-317 aa PF13401 AAA_22 168-254 aa PF23559 WHD_DRP 401-468 aa

XP_057763819.1

838 aa

419

838

Vps16_N

Vps16_C

PF04841 Vps16_N 8-403 aa PF04840 Vps16_C 505-817 aa

XP_057763820.1

829 aa

414

829

Vps16_N

Vps16_C

PF04841 Vps16_N 8-403 aa PF04840 Vps16_C 505-822 aa

XP_057763821.1

805 aa

402

805

Vps16_N

Vps16_C

PF04841 Vps16_N 8-403 aa PF04840 Vps16_C 505-790 aa

Protein ID	来源	结构域编号	结构域名称	类型	起始 aa	终止 aa	E-value	Score	说明
`XP_057763815.1`	Pfam	`PF00931`	NB-ARC	Domain	201	353	2.9e-38	131.9	NB-ARC domain
`XP_057763815.1`	Pfam	`PF23559`	WHD_DRP	Domain	438	507	9.9e-20	71.1	Disease resistance protein Winged helix domain
`XP_057763816.1`	Pfam	`PF00931`	NB-ARC	Domain	201	353	2.9e-38	131.9	NB-ARC domain
`XP_057763816.1`	Pfam	`PF23559`	WHD_DRP	Domain	438	507	9.9e-20	71.1	Disease resistance protein Winged helix domain
`XP_057763817.1`	Pfam	`PF00931`	NB-ARC	Domain	154	317	1.9e-42	145.5	NB-ARC domain
`XP_057763817.1`	Pfam	`PF13401`	AAA_22	Domain	168	254	5.1e-07	30.8	AAA domain
`XP_057763817.1`	Pfam	`PF23559`	WHD_DRP	Domain	401	468	1.2e-19	70.9	Disease resistance protein Winged helix domain
`XP_057763818.1`	Pfam	`PF00931`	NB-ARC	Domain	154	317	1.9e-42	145.5	NB-ARC domain
`XP_057763818.1`	Pfam	`PF13401`	AAA_22	Domain	168	254	5.1e-07	30.8	AAA domain
`XP_057763818.1`	Pfam	`PF23559`	WHD_DRP	Domain	401	468	1.2e-19	70.9	Disease resistance protein Winged helix domain
`XP_057763819.1`	Pfam	`PF04841`	Vps16_N	Repeat	8	403	1.6e-83	281.5	Vps16, N-terminal region
`XP_057763819.1`	Pfam	`PF04840`	Vps16_C	Repeat	505	817	7.7e-73	246.1	Vps16, C-terminal region
`XP_057763820.1`	Pfam	`PF04841`	Vps16_N	Repeat	8	403	1.5e-83	281.6	Vps16, N-terminal region
`XP_057763820.1`	Pfam	`PF04840`	Vps16_C	Repeat	505	822	1.6e-67	228.5	Vps16, C-terminal region
`XP_057763821.1`	Pfam	`PF04841`	Vps16_N	Repeat	8	403	1.4e-83	281.7	Vps16, N-terminal region
`XP_057763821.1`	Pfam	`PF04840`	Vps16_C	Repeat	505	790	3.3e-67	227.5	Vps16, C-terminal region

cDNA / CDS / Protein 序列

下载基因组 DNA（仅基因区域）

每个公开基因均提供参考注释对应的转录本序列；蛋白编码基因另提供 CDS 和蛋白序列。非编码 RNA 不适用 CDS 或蛋白下载。真实 accession 的版本后缀予以保留。

cDNA

24 records

下载 cDNA FASTA

XR_009088891.1
ATATGGGCAGGCTTTGAAACTTCATGATTAATCACTCATCTAATCTCATCACATCTCCTATTCTGCTTCTCCCAAAACAACCATGGCAGCAGCTTATGCAACTCTTGTTTCTGCTATGAATATCATAAACAACCTTCACCATCATCCTCGCCCTCCTGTTTCTCTCCACGTTAAACAAGTTGATTCTCTCACTCAAAAGATTAATTTCCTGCTCGAATTTCTCCAAGGCTATAACCCCCATCGTGACTACTCCAAAGAAG...

XR_009088890.1
TACAATCGTATTTATGCCAAACGGCTTTAATTCATTCACACTCTCATCTTCATTCTCACTCATCTCATCTCATCTCATCTCATTCTCTGCAACTCCCAAAACAACCATGGCAGCAGCTTATGCAACTCTAGTTTCTGCTATGAATATCATAAACAACCTTCACCATCATCCTACCCCTCCTATTTCTCTCCCCATTCAGCAGCTAATGTCTCTCACTCAAAAGATTAATTTCCTGCTCGAATTTCTGGAGGGGTATAACC...

CDS

7 records

下载 CDS FASTA

LOC130985064
ATGGCAGCAGCTTATGCAACTCTTGTTTCTGCTATGAATATCATAAACAACCTTCACCATCATCCTCGCCCTCCTGTTTCTCTCCACGTTAAACAAGTTGATTCTCTCACTCAAAAGATTAATTTCCTGCTCGAATTTCTCCAAGGCTATAACCCCCATCGTGACTACTCCAAAGAAGCGGATCCATTGGAGTGTCGCATTGCAGATGCAGCTTATGCGGCCGAACACGCCATTGAATCTCATATTTTCGACCAGTTCAC...

Protein

7 records

下载 Protein FASTA

XP_057763815.1
MPNGFNSFTLSSSFSLISSHLISFSATPKTTMAAAYATLVSAMNIINNLHHHPTPPISLPIQQLMSLTQKINFLLEFLEGYNPHRGYSKQADPLECRIADAAYAAEHAIESHIFDQFTNNGENMSSDGLCEALEKVIKVMASIEEEAKQIQGRVGVQHQLHTKSTPSDSSRSSSIPQQSSMVGADNAKFQMKDMLTSDVLHLQIIPIVGMGGSGKTTLARTVYEERLIKDHFHICAWATISQEYITREILAAVLHQFNIW...

XP_057763816.1
MPNGFNSFTLSSSFSLISSHLISFSATPKTTMAAAYATLVSAMNIINNLHHHPTPPISLPIQQLMSLTQKINFLLEFLEGYNPHRGYSKQADPLECRIADAAYAAEHAIESHIFDQFTNNGENMSSDGLCEALEKVIKVMASIEEEAKQIQGRVGVQHQLHTKSTPSDSSRSSSIPQQSSMVGADNAKFQMKDMLTSDVLHLQIIPIVGMGGSGKTTLARTVYEERLIKDHFHICAWATISQEYITREILAAVLHQFNIW...

上下游序列下载

upstream/downstream 最大各 10000 bp。负链基因会自动反向互补，使最终 FASTA 为转录方向 5′→3′。

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