Gene Card 基因详情

支持统一新 ID、NCBI Gene ID、GeneID、locus_tag、Transcript ID、Protein ID，以及已人工核对的文献基因名查询。页面会先解析到丹参统一基因，再整合坐标、序列、注释、亚细胞定位、EMS 突变和下载入口。

当前解析结果

`SmilChr05G001165`

染色体: Chr05 坐标: 9774259-9788194 链方向: - 类型: protein_coding

基本信息

统一新 Gene ID	`SmilChr05G001165`
名称	SmilChr05G001165
描述	transcription initiation factor IIF subunit alpha-like
染色体 / SeqID	Chr05 / NC_080391.1
坐标与链方向	9774259 - 9788194 / `-`

ID 对照

NCBI Gene ID	`LOC130985846`
GeneID	`130985846`
locus_tag	`-`
NCBI Name	`LOC130985846`
原始查询	`SmilChr05G001165`

查看完整 ID 对照表

功能注释

来源	GFF basic annotation; NCBI GO GAF; KEGG smil; Pfam-A/HMMER; Arabidopsis/Rice homolog
描述	transcription initiation factor IIF subunit alpha-like
产物	transcription initiation factor IIF subunit alpha-like
Arabidopsis	`AT4G12610` (RAP74) transcription initiation factor IIF subunit alpha RAP74 [Source:NCBI gene (formerly Entrezgene);Acc:826874] identity=66.2; qcov=1.01; evalue=0; confidence=high
Rice	`Os10g0188100` - identity=63.2; qcov=1.01; evalue=0; confidence=high
GO	GO:0001096 (TFIIF-class transcription factor complex binding); GO:0003677 (DNA binding); GO:0005634 (nucleus); GO:0005674 (transcription factor TFIIF complex); GO:0006367 (transcription initiation at RNA polymerase II promoter); GO:0016251 (RNA polymerase II general transcription initiation factor activity); GO:0032968 (positive regulation of transcription elongation by RNA polymerase II)
KEGG	smil03022 (Basal transcription factors - Salvia miltiorrhiza (redroot sage))
Pfam	PF05793 (TFIIF_alpha)
InterPro	-
eggNOG	-
NR	GeneID:130985846

打开注释模块

亚细胞定位 Top 5

Rank	定位位置	中文	Score	Protein ID
1	Nucleus	细胞核	1.00000	`XP_057764988.1`
2	Nucleoplasm	核质	1.00000	`XP_057764988.1`
3	Chromosome	染色体	0.40000	`XP_057764988.1`
4	Nuclear body	核内小体	0.40000	`XP_057764988.1`
5	Chromatin	染色质	0.20000	`XP_057764988.1`

进入亚细胞定位查询

EMS 诱变突变

下表显示该基因在丹参 EMS 材料中的前 12 个候选突变；完整结果可进入 EMS 突变库继续筛选或下载。

位置	REF	ALT	类型	影响	携带材料数	材料
`Chr5:9783122`	`G`	`A`	EMS_canonical_SNV	CDS_variant	2	B1,B2
`Chr5:9783653`	`C`	`CT`	1bp_insertion	frameshift_variant	2	B13,B40
`Chr5:9783653`	`C`	`CT`	1bp_insertion	CDS_variant	2	B13,B40
`Chr5:9783657`	`C`	`T`	EMS_canonical_SNV	CDS_variant	1	B37
`Chr5:9783982`	`G`	`A`	EMS_canonical_SNV	CDS_variant	1	B72
`Chr5:9783999`	`C`	`T`	EMS_canonical_SNV	CDS_variant	1	B49
`Chr5:9785631`	`G`	`A`	EMS_canonical_SNV	CDS_variant	5	B19,B42,B43,B44,B62
`Chr5:9785654`	`G`	`A`	EMS_canonical_SNV	CDS_variant	4	A25,B101,B136,C39
`Chr5:9785800`	`C`	`T`	EMS_canonical_SNV	CDS_variant	1	C2
`Chr5:9786880`	`C`	`T`	EMS_canonical_SNV	CDS_variant	1	D11

进入 EMS 突变库

预测功能结构域

结构域结果来自 Pfam-A HMM 数据库和 HMMER hmmscan；位置为对应蛋白序列上的氨基酸坐标。

XP_057764988.1

540 aa

270

540

TFIIF_alpha

PF05793 TFIIF_alpha 48-538 aa

XP_057764989.1

540 aa

270

540

TFIIF_alpha

PF05793 TFIIF_alpha 48-538 aa

XP_057764990.1

540 aa

270

540

TFIIF_alpha

PF05793 TFIIF_alpha 48-538 aa

Protein ID	来源	结构域编号	结构域名称	类型	起始 aa	终止 aa	E-value	Score	说明
`XP_057764988.1`	Pfam	`PF05793`	TFIIF_alpha	Family	48	538	1.4e-140	470.1	Transcription initiation factor IIF, alpha subunit (TFIIF-alpha)
`XP_057764989.1`	Pfam	`PF05793`	TFIIF_alpha	Family	48	538	1.4e-140	470.1	Transcription initiation factor IIF, alpha subunit (TFIIF-alpha)
`XP_057764990.1`	Pfam	`PF05793`	TFIIF_alpha	Family	48	538	1.4e-140	470.1	Transcription initiation factor IIF, alpha subunit (TFIIF-alpha)

cDNA / CDS / Protein 序列

下载基因组 DNA（仅基因区域）

每个公开基因均提供参考注释对应的转录本序列；蛋白编码基因另提供 CDS 和蛋白序列。非编码 RNA 不适用 CDS 或蛋白下载。真实 accession 的版本后缀予以保留。

cDNA

3 records

下载 cDNA FASTA

XM_057909005.1
ATACAAGAACATCGTCTATCTTTCAAGAACCACACAAAGGTGACAAAACAAAAGCCCCATTCCTTCCCCCGGCCTAAAATTACCAAGGTTTCGAAAAGGGCCTACTTTGCATGACAAACACGCGATTTGGAGAGTGAACAAATTTGGATTTATCTTTGGAACGAAGCGTTTATCTTTGATTGCGAGGAAATTTGCAACGGGAGAAGATGTCGATAGACTTGATATTGAAGCCGTCGTGCGGCGGTTGTGGATCGACGGAT...

XM_057909006.1
CTATCTTTCAAGAACCACACAAAGGTGACAAAACAAAAGCCCCATTCCTTCCCCCGGCCTAAAATTACCAAGGTTTCGAAAAGGGCCTACTTTGCATGACAAACACGCGATTTGGAGAGTGAACAAATTTGGATTTATCTTTGGAACGAAGCGTTTATCTTTGATTGCGAGGTTCAATTCAAAATTTTCAGCACCAATTAAACTGAATTGAAATTTGCAACGGGAGAAGATGTCGATAGACTTGATATTGAAGCCGTCGT...

CDS

3 records

下载 CDS FASTA

LOC130985846
ATGTCGATAGACTTGATATTGAAGCCGTCGTGCGGCGGTTGTGGATCGACGGATGAGCTATACGGAAGCACGTGCAAGCACCTGACGCTTTGCGTGAATTGTGGCAAATCGATGGCCGAGAAACGTTTAAAGTGCTACGAGTGCGGCACCCCCATCACTCGCCTGATTAGGGAATACAATGTGCGTGCTAGTTCCAGCAACGACAAAAACTACTTCATTGCAAGATTTGCCTCAGGATTACCAAGTTTTAAGAAGAAGAA...

Protein

3 records

下载 Protein FASTA

XP_057764988.1
MSIDLILKPSCGGCGSTDELYGSTCKHLTLCVNCGKSMAEKRLKCYECGTPITRLIREYNVRASSSNDKNYFIARFASGLPSFKKKNENKWSLQKEGLQGRQVTDTLREKFKNKPWLLEDETGQFQYHGHLEGTQTATYYLLMLQGKEFVAIPAGSWYNFTKVAQYKQLTLEEAEEKMKNRRRTADGYERWMMKAAHNGAAAFGEVEKFDDREVAGGGGGGKGRKKATGDDDEANVSDRGDEDEEEEVARKNRLGLNKKS...

XP_057764989.1
MSIDLILKPSCGGCGSTDELYGSTCKHLTLCVNCGKSMAEKRLKCYECGTPITRLIREYNVRASSSNDKNYFIARFASGLPSFKKKNENKWSLQKEGLQGRQVTDTLREKFKNKPWLLEDETGQFQYHGHLEGTQTATYYLLMLQGKEFVAIPAGSWYNFTKVAQYKQLTLEEAEEKMKNRRRTADGYERWMMKAAHNGAAAFGEVEKFDDREVAGGGGGGKGRKKATGDDDEANVSDRGDEDEEEEVARKNRLGLNKKS...

上下游序列下载

upstream/downstream 最大各 10000 bp。负链基因会自动反向互补，使最终 FASTA 为转录方向 5′→3′。

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