Gene Card 基因详情

支持统一新 ID、NCBI Gene ID、GeneID、locus_tag、Transcript ID、Protein ID，以及已人工核对的文献基因名查询。页面会先解析到丹参统一基因，再整合坐标、序列、注释、亚细胞定位、EMS 突变和下载入口。

当前解析结果

`SmilChr05G001547`

染色体: Chr05 坐标: 14161465-14164281 链方向: - 类型: protein_coding

基本信息

统一新 Gene ID	`SmilChr05G001547`
名称	SmilChr05G001547
描述	uncharacterized SmilChr05G001547
染色体 / SeqID	Chr05 / NC_080391.1
坐标与链方向	14161465 - 14164281 / `-`

ID 对照

NCBI Gene ID	`LOC131025544`
GeneID	`131025544`
locus_tag	`-`
NCBI Name	`LOC131025544`
原始查询	`SmilChr05G001547`

查看完整 ID 对照表

功能注释

来源	GFF basic annotation; NCBI GO GAF; Pfam-A/HMMER; Arabidopsis/Rice homolog
描述	hit=Os04g0191000; species=rice; confidence=low
产物	hit=Os04g0191000; species=rice; confidence=low
Arabidopsis	`AT3G01410` (AT3G01410) Polynucleotidyl transferase, ribonuclease H-like superfamily protein [Source:NCBI gene (formerly Entrezgene);Acc:821319] identity=39.6; qcov=0.112; evalue=9.98e-11; confidence=weak
Rice	`Os04g0191000` - identity=27.8; qcov=0.409; evalue=8.19e-33; confidence=low
GO	GO:0003676 (nucleic acid binding); GO:0004523 (RNA-DNA hybrid ribonuclease activity); GO:0015074 (DNA integration)
KEGG	-
Pfam	PF00075 (RNase_H); PF00078 (RVT_1); PF00665 (rve); PF13456 (RVT_3); PF17917 (RT_RNaseH); PF17919 (RT_RNaseH_2); PF17921 (Integrase_H2C2); PF24626 (SH3_Tf2-1); PF29688 (TnsB_C); PF31073 (DUF8803)
InterPro	-
eggNOG	-
NR	GeneID:131025544

打开注释模块

亚细胞定位 Top 5

Rank	定位位置	中文	Score	Protein ID
1	Cytoplasm	细胞质	1.00000	`XP_057811321.1`
2	Cytosol	细胞质基质	0.60000	`XP_057811321.1`
3	Nucleus	细胞核	0.00000	`XP_057811321.1`
4	Chromosome	染色体	0.00000	`XP_057811321.1`
5	Centromere	着丝粒	0.00000	`XP_057811321.1`

进入亚细胞定位查询

EMS 诱变突变

下表显示该基因在丹参 EMS 材料中的前 12 个候选突变；完整结果可进入 EMS 突变库继续筛选或下载。

位置	REF	ALT	类型	影响	携带材料数	材料
`Chr5:14161474`	`G`	`A`	EMS_canonical_SNV	CDS_variant	2	A5,B33
`Chr5:14161479`	`G`	`A`	EMS_canonical_SNV	stop_gained	6	A20,B124,B52,C11,C36,C43
`Chr5:14161491`	`G`	`A`	EMS_canonical_SNV	CDS_variant	5	B19,B27,B81,C64,D4
`Chr5:14161498`	`C`	`T`	EMS_canonical_SNV	stop_gained	1	C46
`Chr5:14161498`	`C`	`T`	EMS_canonical_SNV	CDS_variant	1	C46
`Chr5:14161504`	`G`	`A`	EMS_canonical_SNV	CDS_variant	2	C17,C59
`Chr5:14161517`	`C`	`T`	EMS_canonical_SNV	CDS_variant	3	B58,C32,C66
`Chr5:14161520`	`C`	`T`	EMS_canonical_SNV	CDS_variant	5	B138,B25,B46,C36,C63
`Chr5:14161522`	`G`	`A`	EMS_canonical_SNV	CDS_variant	1	C60
`Chr5:14161524`	`C`	`T`	EMS_canonical_SNV	CDS_variant	1	C17
`Chr5:14161526`	`G`	`A`	EMS_canonical_SNV	CDS_variant	1	B56
`Chr5:14161532`	`C`	`T`	EMS_canonical_SNV	CDS_variant	1	C17

进入 EMS 突变库

预测功能结构域

结构域结果来自 Pfam-A HMM 数据库和 HMMER hmmscan；位置为对应蛋白序列上的氨基酸坐标。

XP_057811321.1

904 aa

452

904

RVT_1

RT_RNaseH_2

RT_RNaseH

RNase_H

RVT_3

Integrase_H2C2

rve

TnsB_C

DUF8803

SH3_Tf2-1

PF00078 RVT_1 4-125 aa PF17919 RT_RNaseH_2 158-256 aa PF17917 RT_RNaseH 187-285 aa PF00075 RNase_H 333-412 aa PF13456 RVT_3 336-454 aa PF17921 Integrase_H2C2 545-601 aa PF00665 rve 620-716 aa PF29688 TnsB_C 677-737 aa PF31073 DUF8803 839-902 aa PF24626 SH3_Tf2-1 842-903 aa

Protein ID	来源	结构域编号	结构域名称	类型	起始 aa	终止 aa	E-value	Score	说明
`XP_057811321.1`	Pfam	`PF00078`	RVT_1	Domain	4	125	4e-22	79.8	Reverse transcriptase (RNA-dependent DNA polymerase)
`XP_057811321.1`	Pfam	`PF17919`	RT_RNaseH_2	Domain	158	256	3.4e-26	91.9	RNase H-like domain found in reverse transcriptase
`XP_057811321.1`	Pfam	`PF17917`	RT_RNaseH	Domain	187	285	8.6e-22	78.0	RNase H-like domain found in reverse transcriptase
`XP_057811321.1`	Pfam	`PF00075`	RNase_H	Domain	333	412	1.7e-08	35.4	RNase H
`XP_057811321.1`	Pfam	`PF13456`	RVT_3	Domain	336	454	2.6e-18	66.8	Reverse transcriptase-like
`XP_057811321.1`	Pfam	`PF17921`	Integrase_H2C2	Domain	545	601	5.8e-10	39.9	Integrase zinc binding domain
`XP_057811321.1`	Pfam	`PF00665`	rve	Domain	620	716	1.1e-18	68.1	Integrase core domain
`XP_057811321.1`	Pfam	`PF29688`	TnsB_C	Domain	677	737	2.2e-09	38.0	TnsB C-terminal domain
`XP_057811321.1`	Pfam	`PF31073`	DUF8803	Domain	839	902	8.6e-27	93.5	Domain of unknown function (DUF8803)
`XP_057811321.1`	Pfam	`PF24626`	SH3_Tf2-1	Domain	842	903	9e-08	32.8	Tf2-1-like, SH3 domain

cDNA / CDS / Protein 序列

下载基因组 DNA（仅基因区域）

每个公开基因均提供参考注释对应的转录本序列；蛋白编码基因另提供 CDS 和蛋白序列。非编码 RNA 不适用 CDS 或蛋白下载。真实 accession 的版本后缀予以保留。

cDNA

1 records

下载 cDNA FASTA

XM_057955338.1
ATGCTAGTGGACGCGACAGCAGGACATGAATTGATGAGTTTCATGGACGCATACTCGGGATACCATCAAATCCGGATGCACCCTGATGATGAGGAGAAGACGTCCTTCATGACCAGCATAGCGCTATTTTGCTACAAATATATGCCATTCAGATTGAAGAATGCAGGAGCAACATATCAACGCCTGGTCAATCGGATGTTTGCAAGCTTGCTGGGCCAAACAATGGAAGTCTACATCGATGACATGTTGGTCAAGTCCAT...

CDS

1 records

下载 CDS FASTA

LOC131025544
ATGCTAGTGGACGCGACAGCAGGACATGAATTGATGAGTTTCATGGACGCATACTCGGGATACCATCAAATCCGGATGCACCCTGATGATGAGGAGAAGACGTCCTTCATGACCAGCATAGCGCTATTTTGCTACAAATATATGCCATTCAGATTGAAGAATGCAGGAGCAACATATCAACGCCTGGTCAATCGGATGTTTGCAAGCTTGCTGGGCCAAACAATGGAAGTCTACATCGATGACATGTTGGTCAAGTCCAT...

Protein

1 records

下载 Protein FASTA

XP_057811321.1
MLVDATAGHELMSFMDAYSGYHQIRMHPDDEEKTSFMTSIALFCYKYMPFRLKNAGATYQRLVNRMFASLLGQTMEVYIDDMLVKSIRARDHIDHLRETFEILRKYNMKLNPTKCSFGVNAGKFLAGRIAALSRFISRSSEKCHLFFNTLRKSKTFEWTEECEEALKQLKQYLTSSPLLAKPKNHETLLVYLAVSDTAVSAVLVREEEGKQSPIYYVSKSLLEAETRYSQLEKLALALVHAARKLRPYFQCHPIVVATTY...

上下游序列下载

upstream/downstream 最大各 10000 bp。负链基因会自动反向互补，使最终 FASTA 为转录方向 5′→3′。

下载 upstream 下载 downstream 下载 upstream + gene + downstream

JBrowse

跳转到基因组浏览器