Gene Card 基因详情

支持统一新 ID、NCBI Gene ID、GeneID、locus_tag、Transcript ID、Protein ID，以及已人工核对的文献基因名查询。页面会先解析到丹参统一基因，再整合坐标、序列、注释、亚细胞定位、EMS 突变和下载入口。

当前解析结果

`SmilChr05G002112`

染色体: Chr05 坐标: 22128587-22156014 链方向: - 类型: protein_coding

基本信息

统一新 Gene ID	`SmilChr05G002112`
名称	SmilChr05G002112
描述	DNA repair protein RAD50
染色体 / SeqID	Chr05 / NC_080391.1
坐标与链方向	22128587 - 22156014 / `-`

ID 对照

NCBI Gene ID	`LOC130986737`
GeneID	`130986737`
locus_tag	`-`
NCBI Name	`LOC130986737`
原始查询	`SmilChr05G002112`

查看完整 ID 对照表

功能注释

来源	GFF basic annotation; NCBI GO GAF; KEGG smil; Pfam-A/HMMER; Arabidopsis/Rice homolog
描述	DNA repair protein RAD50
产物	DNA repair protein RAD50
Arabidopsis	`AT2G31970` (RAD50) DNA repair-recombination protein (RAD50) [Source:NCBI gene (formerly Entrezgene);Acc:817756] identity=60.4; qcov=0.987; evalue=0; confidence=high
Rice	`Os02g0497500` (Rad50) - identity=58; qcov=0.599; evalue=0; confidence=medium
GO	GO:0000722 (telomere maintenance via recombination); GO:0000794 (condensed nuclear chromosome); GO:0003691 (double-stranded telomeric DNA binding); GO:0005634 (nucleus); GO:0006302 (double-strand break repair); GO:0007004 (telomere maintenance via telomerase); GO:0016887 (ATP hydrolysis activity); GO:0030870 (Mre11 complex); GO:0032508 (GO:0032508); GO:0043047 (single-stranded telomeric DNA binding); GO:0051880 (G-quadruplex DNA binding); GO:0070192 (chromosome organization involved in meiotic cell cycle); GO:0090305 (GO:0090305)
KEGG	smil03440 (Homologous recombination - Salvia miltiorrhiza (redroot sage)); smil03450 (Non-homologous end-joining - Salvia miltiorrhiza (redroot sage))
Pfam	PF04423 (Rad50_zn_hook); PF13476 (AAA_23); PF13558 (SbcC_Walker_B)
InterPro	-
eggNOG	-
NR	GeneID:130986737

打开注释模块

亚细胞定位 Top 5

Rank	定位位置	中文	Score	Protein ID
1	Nucleus	细胞核	1.00000	`XP_057766215.1`
2	Chromosome	染色体	1.00000	`XP_057766215.1`
3	Centromere	着丝粒	0.40000	`XP_057766215.1`
4	Nucleoplasm	核质	0.40000	`XP_057766215.1`
5	Telomere	端粒	0.20000	`XP_057766215.1`

进入亚细胞定位查询

EMS 诱变突变

下表显示该基因在丹参 EMS 材料中的前 12 个候选突变；完整结果可进入 EMS 突变库继续筛选或下载。

位置	REF	ALT	类型	影响	携带材料数	材料
`Chr5:22128935`	`TCC`	`T`	2bp_deletion	frameshift_variant	3	B120,B79,D8
`Chr5:22128935`	`TCC`	`T`	2bp_deletion	CDS_variant	3	B120,B79,D8
`Chr5:22128948`	`G`	`A`	EMS_canonical_SNV	CDS_variant	3	B120,B71,D8
`Chr5:22129217`	`C`	`T`	EMS_canonical_SNV	CDS_variant	4	B60,B9,C19,C22
`Chr5:22129239`	`C`	`T`	EMS_canonical_SNV	CDS_variant	4	B60,B9,C19,C22
`Chr5:22129251`	`C`	`T`	EMS_canonical_SNV	CDS_variant	3	B54,B9,C22
`Chr5:22131661`	`C`	`T`	EMS_canonical_SNV	CDS_variant	1	B105
`Chr5:22134893`	`G`	`A`	EMS_canonical_SNV	CDS_variant	1	C9
`Chr5:22135311`	`C`	`T`	EMS_canonical_SNV	CDS_variant	3	B107,B27,C28
`Chr5:22135332`	`G`	`A`	EMS_canonical_SNV	CDS_variant	1	D10
`Chr5:22138483`	`C`	`T`	EMS_canonical_SNV	CDS_variant	3	A1,B75,C74
`Chr5:22140566`	`C`	`T`	EMS_canonical_SNV	CDS_variant	1	A22

进入 EMS 突变库

预测功能结构域

结构域结果来自 Pfam-A HMM 数据库和 HMMER hmmscan；位置为对应蛋白序列上的氨基酸坐标。

XP_057766215.1

1316 aa

658

1316

AAA_23

Rad50_zn_hook

SbcC_Walker_B

PF13476 AAA_23 6-250 aa PF04423 Rad50_zn_hook 686-723 aa PF13558 SbcC_Walker_B 1189-1258 aa

XP_057766216.1

1209 aa

604

1209

Rad50_zn_hook

SbcC_Walker_B

PF04423 Rad50_zn_hook 579-616 aa PF13558 SbcC_Walker_B 1082-1151 aa

XP_057766217.1

1207 aa

604

1207

Rad50_zn_hook

SbcC_Walker_B

PF04423 Rad50_zn_hook 577-614 aa PF13558 SbcC_Walker_B 1080-1149 aa

Protein ID	来源	结构域编号	结构域名称	类型	起始 aa	终止 aa	E-value	Score	说明
`XP_057766215.1`	Pfam	`PF13476`	AAA_23	Domain	6	250	1.6e-37	130.8	AAA domain
`XP_057766215.1`	Pfam	`PF04423`	Rad50_zn_hook	Motif	686	723	3.4e-08	33.9	Rad50 zinc hook motif
`XP_057766215.1`	Pfam	`PF13558`	SbcC_Walker_B	Domain	1189	1258	1.9e-06	28.7	SbcC/RAD50-like, Walker B motif
`XP_057766216.1`	Pfam	`PF04423`	Rad50_zn_hook	Motif	579	616	3.1e-06	27.6	Rad50 zinc hook motif
`XP_057766216.1`	Pfam	`PF13558`	SbcC_Walker_B	Domain	1082	1151	1.6e-06	29.0	SbcC/RAD50-like, Walker B motif
`XP_057766217.1`	Pfam	`PF04423`	Rad50_zn_hook	Motif	577	614	3.1e-06	27.6	Rad50 zinc hook motif
`XP_057766217.1`	Pfam	`PF13558`	SbcC_Walker_B	Domain	1080	1149	1.6e-06	29.0	SbcC/RAD50-like, Walker B motif

cDNA / CDS / Protein 序列

下载基因组 DNA（仅基因区域）

每个公开基因均提供参考注释对应的转录本序列；蛋白编码基因另提供 CDS 和蛋白序列。非编码 RNA 不适用 CDS 或蛋白下载。真实 accession 的版本后缀予以保留。

cDNA

3 records

下载 cDNA FASTA

XM_057910232.1
GTGTTCTGGAACAGGCAACGTACTTCTCCCCGCCCTTTTTCATTTCTCAATTAAATTCCCTCTTAAACCCTATTCCGTCGCTCTAGTTCTTCCCCATTTTCCGATGAAGAACCCTTTCTTCGACGAATTCTCAACATGCGGTAATCAACCATCTCTCTAAAAATATACTCCTTCTTCTTCTTCTTCTCTTCTATGCCATCAGGCTCTGTAGAGAAAGCAGTTGGAAAAGATGAGTACTGTCGACAAAATGTTGATAAAGG...

XM_057910233.1
ACGCTATGCTTAGAAAGCGACTATTATTGAGTGCCTGAAAGTAGCCTGTACAGGTGAACTTCCTCCAAATGCACGGTCTGGTCACAGCTTCATTCACGACCCTAAGGTGGCAGGGGAGACAGAGACGAAGGGCCAGATAAAGCTAAGGTTTAAGACGGCAGCAAGGAAGGATGTGGTCTGCATAAGGTCATTTCAGCTCACCCAAAAAGCCACCAAGATGGAGTACAAGGCAATTGAGTCTGTGCTTCAGACGATAAATC...

CDS

3 records

下载 CDS FASTA

LOC130986737
ATGAGTACTGTCGACAAAATGTTGATAAAGGGCATCCGGAGCTTCGACCCGGAAAACAAAAACGTCATCACCTTCTTCAAGCCACTCACTCTCATTGTCGGCCCCAACGGTGCCGGAAAAACCACTATTATTGAGTGCCTGAAAGTAGCCTGTACAGGTGAACTTCCTCCAAATGCACGGTCTGGTCACAGCTTCATTCACGACCCTAAGGTGGCAGGGGAGACAGAGACGAAGGGCCAGATAAAGCTAAGGTTTAAGAC...

LOC130986737
ATGGAGTACAAGGCAATTGAGTCTGTGCTTCAGACGATAAATCCTCAGACTGGACAGAAAGTTTGCCTAAGCTACCGTTGTGCTGATATGGACCGAGAGATTCCAGCTCTTATGGGGGTGTCCAAGGCTGTTCTCGAGAATGTTATATTTGTACACCAGGATGAGGCAAACTGGCCATTGCAAGATCCATCAACACTGAAAAAGAAGTTTGATGATATCTTTTCGGCAACTAGGTACACTAAGGCATTAGAGGTCATCAA...

Protein

3 records

下载 Protein FASTA

XP_057766215.1
MSTVDKMLIKGIRSFDPENKNVITFFKPLTLIVGPNGAGKTTIIECLKVACTGELPPNARSGHSFIHDPKVAGETETKGQIKLRFKTAARKDVVCIRSFQLTQKATKMEYKAIESVLQTINPQTGQKVCLSYRCADMDREIPALMGVSKAVLENVIFVHQDEANWPLQDPSTLKKKFDDIFSATRYTKALEVIKKLHKDQAQEIKTYKLKLDHLQTLKDAAFKLRESIAQYEAKIETLNSQMQELDPKIQNIDREINKTE...

XP_057766216.1
MEYKAIESVLQTINPQTGQKVCLSYRCADMDREIPALMGVSKAVLENVIFVHQDEANWPLQDPSTLKKKFDDIFSATRYTKALEVIKKLHKDQAQEIKTYKLKLDHLQTLKDAAFKLRESIAQYEAKIETLNSQMQELDPKIQNIDREINKTELLLKDLRKLQDQISTKSGERKSKFEDVQKRYSDLDEENEDPDEQLNEWKSKFDERIAILESKISKLLREKMDIEDESRHHENDIAQSIVKIAKLQAAIEAQLSQKDI...

上下游序列下载

upstream/downstream 最大各 10000 bp。负链基因会自动反向互补，使最终 FASTA 为转录方向 5′→3′。

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