Gene Card 基因详情

支持统一新 ID、NCBI Gene ID、GeneID、locus_tag、Transcript ID、Protein ID，以及已人工核对的文献基因名查询。页面会先解析到丹参统一基因，再整合坐标、序列、注释、亚细胞定位、EMS 突变和下载入口。

当前解析结果

`SmilChr05G004056`

染色体: Chr05 坐标: 54519527-54527917 链方向: + 类型: protein_coding

基本信息

统一新 Gene ID	`SmilChr05G004056`
名称	SmilChr05G004056
描述	vacuolar-sorting protein BRO1
染色体 / SeqID	Chr05 / NC_080391.1
坐标与链方向	54519527 - 54527917 / `+`

ID 对照

NCBI Gene ID	`LOC131025119`
GeneID	`131025119`
locus_tag	`-`
NCBI Name	`LOC131025119`
原始查询	`SmilChr05G004056`

查看完整 ID 对照表

功能注释

来源	GFF basic annotation; NCBI GO GAF; KEGG smil; Pfam-A/HMMER; Arabidopsis/Rice homolog
描述	vacuolar-sorting protein BRO1
产物	vacuolar-sorting protein BRO1
Arabidopsis	`AT1G15130` (AT1G15130) Endosomal targeting BRO1-like domain-containing protein [Source:NCBI gene (formerly Entrezgene);Acc:838077] identity=68.2; qcov=0.998; evalue=0; confidence=high
Rice	`Os10g0495300` (OSJNBa0041P03.2) - identity=61.8; qcov=0.977; evalue=0; confidence=high
GO	GO:0005515 (protein binding); GO:0005768 (endosome); GO:0043328 (protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway)
KEGG	smil03250 (Viral life cycle - HIV-1 - Salvia miltiorrhiza (redroot sage)); smil03272 (Virion - Hepatitis viruses - Salvia miltiorrhiza (redroot sage)); smil04144 (Endocytosis - Salvia miltiorrhiza (redroot sage))
Pfam	PF03097 (BRO1); PF13949 (ALIX_LYPXL_bnd)
InterPro	-
eggNOG	-
NR	GeneID:131025119

打开注释模块

亚细胞定位 Top 5

Rank	定位位置	中文	Score	Protein ID
1	Cytoplasm	细胞质	1.00000	`XP_057810716.1`
2	Cytoplasmic vesicle	细胞质囊泡	0.80000	`XP_057810716.1`
3	Clathrin-coated vesicle	Clathrin-coated vesicle	0.60000	`XP_057810716.1`
4	Vesicle	囊泡	0.60000	`XP_057810716.1`
5	Endosome	内体	0.60000	`XP_057810716.1`

进入亚细胞定位查询

EMS 诱变突变

下表显示该基因在丹参 EMS 材料中的前 12 个候选突变；完整结果可进入 EMS 突变库继续筛选或下载。

位置	REF	ALT	类型	影响	携带材料数	材料
`Chr5:54519826`	`TC`	`T`	1bp_deletion	frameshift_variant	82	A1,A13,A2,A21,A22,A6,A7,A8,B101,B104,B114,B119,B121,B122,B129,B135,B137,B140,B19,B23,B26,B27,B29,B34,B35,B38,B39,B4,B47,B48,B5,B52,B54,B57,B61,B63,B65,B67,B73,B76,B78,B79,B8,B82,B83,B86,B88,B89,B90,B91,B92,C12,C17,C18,C26,C29,C3,C30,C31,C36,C38,C39,C41,C44,C45,C50,C51,C52,C53,C56,C65,C72,C75,C78,C80,C84,C87,C9,D1,D11,D4,D5
`Chr5:54519832`	`C`	`T`	EMS_canonical_SNV	CDS_variant	1	D11
`Chr5:54519874`	`GGA`	`G`	2bp_deletion	frameshift_variant	80	A1,A13,A2,A21,A22,A6,A7,A8,B101,B104,B114,B119,B121,B122,B129,B134,B135,B137,B140,B19,B23,B25,B26,B27,B29,B34,B35,B38,B39,B4,B47,B48,B5,B52,B54,B57,B61,B63,B65,B67,B73,B76,B78,B79,B8,B82,B83,B86,B88,B89,B90,B91,C17,C18,C26,C29,C3,C30,C31,C36,C38,C41,C44,C45,C50,C52,C53,C56,C65,C72,C75,C78,C80,C84,C87,C9,D1,D11,D4,D5
`Chr5:54519916`	`GA`	`G`	1bp_deletion	frameshift_variant	1	A24
`Chr5:54519916`	`GA`	`G`	1bp_deletion	CDS_variant	1	A24
`Chr5:54519931`	`C`	`T`	EMS_canonical_SNV	CDS_variant	1	B15
`Chr5:54520703`	`C`	`T`	EMS_canonical_SNV	CDS_variant	2	B18,B64
`Chr5:54520755`	`AG`	`A`	1bp_deletion	frameshift_variant	83	A1,A13,A2,A22,A6,A7,A8,B101,B104,B114,B119,B121,B122,B129,B134,B135,B137,B140,B19,B23,B25,B26,B27,B29,B34,B35,B38,B39,B47,B48,B52,B54,B57,B61,B63,B65,B73,B76,B78,B79,B8,B82,B83,B86,B88,B89,B90,B91,B92,C12,C17,C18,C26,C29,C3,C30,C31,C36,C38,C39,C41,C44,C45,C50,C51,C52,C53,C55,C56,C65,C70,C72,C75,C78,C80,C84,C86,C87,C9,D1,D11,D4,D5
`Chr5:54522030`	`C`	`T`	EMS_canonical_SNV	CDS_variant	5	A14,A24,B46,B50,D3
`Chr5:54522175`	`G`	`A`	EMS_canonical_SNV	CDS_variant	1	B101
`Chr5:54522185`	`C`	`T`	EMS_canonical_SNV	CDS_variant	2	B102,B85
`Chr5:54522187`	`G`	`A`	EMS_canonical_SNV	CDS_variant	1	C62

进入 EMS 突变库

预测功能结构域

结构域结果来自 Pfam-A HMM 数据库和 HMMER hmmscan；位置为对应蛋白序列上的氨基酸坐标。

XP_057810716.1

871 aa

436

871

BRO1

ALIX_LYPXL_bnd

PF03097 BRO1 13-392 aa PF13949 ALIX_LYPXL_bnd 429-714 aa

Protein ID	来源	结构域编号	结构域名称	类型	起始 aa	终止 aa	E-value	Score	说明
`XP_057810716.1`	Pfam	`PF03097`	BRO1	Repeat	13	392	4.3e-110	368.9	BRO1-like domain
`XP_057810716.1`	Pfam	`PF13949`	ALIX_LYPXL_bnd	Domain	429	714	4e-74	250.3	ALIX V-shaped domain binding to HIV

cDNA / CDS / Protein 序列

下载基因组 DNA（仅基因区域）

每个公开基因均提供参考注释对应的转录本序列；蛋白编码基因另提供 CDS 和蛋白序列。非编码 RNA 不适用 CDS 或蛋白下载。真实 accession 的版本后缀予以保留。

cDNA

1 records

下载 cDNA FASTA

XM_057954733.1
CTTGCTTTCCAACTAAGCAACGATGCATCGATATAAAATTATTAAAAGAAACGCTAAATAAAATTTAGATAAAATCCTTTTTTCTCAGGCGAAATTTAGAGAAAAACCTTACCGATTCACAAATTTTATCAAATCCACAACGATCATCATCATCAGCTCATCACAGAGCTTTTCTGTCCAAATAAGTTTCATTATCGTCTACAGCTGTTATCCGATCAATCGCCGCCGGTAATGGCATCGGCGTCGTCGACCACTCTCAC...

CDS

1 records

下载 CDS FASTA

LOC131025119
ATGGCATCGGCGTCGTCGACCACTCTCACCAACGTCATGCTGGCGATCCTCGAGAAGAAGACCAACTCTCTCGACCTCTACCGGCCGCTGCGCAACTTCATCGTGATCAATTACTCGGAGCGCGAGGCGCAGAATCTGGAGGATGATCTCGAGACTCTGAAGCAGCTCCGCTCCGACGTCGAGCGCGGCGGGGGATCCGACTCGCTCTCAGCTCGCCGCGACCTCCTGCAGAATTACTATAAAGCCCTGTGCGCGGTGGA...

Protein

1 records

下载 Protein FASTA

XP_057810716.1
MASASSTTLTNVMLAILEKKTNSLDLYRPLRNFIVINYSEREAQNLEDDLETLKQLRSDVERGGGSDSLSARRDLLQNYYKALCAVESRFPISPDKDHVNTVSFTWFDAFKNRQKASQQNIHLEKAAVLFNLGAVHSQMGLSFDRSSVEGRRQASHSFIAAAGAFAFLRDNVAMKASVGSSTTLDISVECVGMLERLMLAQAQECVYENTIAKGSSAGVCAKISRQAGLFYEEALAAVNVTPLNQHVDKAWIAHVHLKAA...

上下游序列下载

upstream/downstream 最大各 10000 bp。负链基因会自动反向互补，使最终 FASTA 为转录方向 5′→3′。

下载 upstream 下载 downstream 下载 upstream + gene + downstream

JBrowse

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