当前解析结果
SmilChr06G000311| 统一新 Gene ID | SmilChr06G000311 |
|---|---|
| 名称 | SmilChr06G000311 |
| 描述 | leucine--tRNA ligase, cytoplasmic-like |
| 染色体 / SeqID | Chr06 / NC_080392.1 |
| 坐标与链方向 | 2239284 - 2243370 / + |
| NCBI Gene ID | LOC130987842 |
|---|---|
| GeneID | 130987842 |
| locus_tag | - |
| NCBI Name | LOC130987842 |
| 原始查询 | SmilChr06G000311 |
| 来源 | GFF basic annotation; NCBI GO GAF; KEGG smil; Pfam-A/HMMER; Arabidopsis/Rice homolog |
|---|---|
| 描述 | leucine--tRNA ligase, cytoplasmic-like |
| 产物 | leucine--tRNA ligase%2C cytoplasmic-like |
| Arabidopsis | AT1G09620 (AT1G09620)ATP binding/leucine-tRNA ligases/aminoacyl-tRNA ligase [Source:NCBI gene (formerly Entrezgene);Acc:837489] identity=70.4; qcov=1; evalue=0; confidence=high |
| Rice | Os09g0503400- identity=72.8; qcov=0.998; evalue=0; confidence=high |
| GO | GO:0002161 (aminoacyl-tRNA deacylase activity); GO:0004823 (leucine-tRNA ligase activity); GO:0005524 (ATP binding); GO:0006429 (leucyl-tRNA aminoacylation) |
| KEGG | smil00970 (Aminoacyl-tRNA biosynthesis - Salvia miltiorrhiza (redroot sage)) |
| Pfam | PF00133 (tRNA-synt_1); PF01406 (tRNA-synt_1e); PF08264 (Anticodon_1); PF09334 (tRNA-synt_1g); PF24810 (RBD_LARS1) |
| InterPro | - |
| eggNOG | - |
| NR | GeneID:130987842 |
| Rank | 定位位置 | 中文 | Score | Protein ID |
|---|---|---|---|---|
| 1 | Cytoplasm | 细胞质 | 1.00000 | XP_057767522.1 |
| 2 | Cytosol | 细胞质基质 | 1.00000 | XP_057767522.1 |
| 3 | Mitochondrion | 线粒体 | 0.40000 | XP_057767521.1 |
| 4 | Chloroplast | 叶绿体 | 0.20000 | XP_057767521.1 |
| 5 | Nucleus | 细胞核 | 0.00000 | XP_057767522.1 |
下表显示该基因在丹参 EMS 材料中的前 12 个候选突变；完整结果可进入 EMS 突变库继续筛选或下载。
| 位置 | REF | ALT | 类型 | 影响 | 携带材料数 | 材料 |
|---|---|---|---|---|---|---|
Chr6:2239293 |
TAC |
T |
2bp_deletion | frameshift_variant | 7 | A10,A24,B115,B20,B99,C29,C30 |
Chr6:2239324 |
C |
CT |
1bp_insertion | frameshift_variant | 2 | B15,C1 |
Chr6:2239324 |
C |
CT |
1bp_insertion | CDS_variant | 2 | B15,C1 |
Chr6:2239356 |
GC |
G |
1bp_deletion | frameshift_variant | 29 | A8,B10,B103,B111,B115,B139,B14,B32,B37,B38,B39,B48,B53,B54,B69,B71,B76,B8,B97,C1,C20,C34,C38,C43,C49,C54,C59,C85,C9 |
Chr6:2239939 |
C |
T |
EMS_canonical_SNV | CDS_variant | 1 | B16 |
Chr6:2239972 |
G |
A |
EMS_canonical_SNV | CDS_variant | 1 | A23 |
Chr6:2240217 |
C |
T |
EMS_canonical_SNV | CDS_variant | 4 | B17,B4,B58,B63 |
Chr6:2240249 |
G |
A |
EMS_canonical_SNV | CDS_variant | 1 | B104 |
Chr6:2240272 |
G |
A |
EMS_canonical_SNV | CDS_variant | 1 | C46 |
Chr6:2240298 |
C |
T |
EMS_canonical_SNV | CDS_variant | 3 | B100,B101,B85 |
Chr6:2240662 |
G |
A |
EMS_canonical_SNV | CDS_variant | 3 | B9,B94,C43 |
Chr6:2240808 |
G |
A |
EMS_canonical_SNV | CDS_variant | 1 | D3 |
结构域结果来自 Pfam-A HMM 数据库和 HMMER hmmscan；位置为对应蛋白序列上的氨基酸坐标。
XP_057767521.1PF00133
tRNA-synt_1 91-174 aa
PF09334
tRNA-synt_1g 121-180 aa
PF00133
tRNA-synt_1 267-827 aa
PF09334
tRNA-synt_1g 735-833 aa
PF01406
tRNA-synt_1e 741-817 aa
PF08264
Anticodon_1 867-990 aa
PF24810
RBD_LARS1 1016-1093 aa
XP_057767522.1PF00133
tRNA-synt_1 22-105 aa
PF09334
tRNA-synt_1g 52-111 aa
PF00133
tRNA-synt_1 198-758 aa
PF09334
tRNA-synt_1g 666-764 aa
PF01406
tRNA-synt_1e 672-748 aa
PF08264
Anticodon_1 798-921 aa
PF24810
RBD_LARS1 947-1024 aa
| Protein ID | 来源 | 结构域编号 | 结构域名称 | 类型 | 起始 aa | 终止 aa | E-value | Score | 说明 |
|---|---|---|---|---|---|---|---|---|---|
XP_057767521.1 |
Pfam | PF00133 |
tRNA-synt_1 | Domain | 91 | 174 | 3.5e-29 | 102.5 | tRNA synthetases class I (I, L, M and V) |
XP_057767521.1 |
Pfam | PF09334 |
tRNA-synt_1g | Family | 121 | 180 | 4.8e-16 | 59.2 | tRNA synthetases class I (M) |
XP_057767521.1 |
Pfam | PF00133 |
tRNA-synt_1 | Domain | 267 | 827 | 3.5e-29 | 102.5 | tRNA synthetases class I (I, L, M and V) |
XP_057767521.1 |
Pfam | PF09334 |
tRNA-synt_1g | Family | 735 | 833 | 4.8e-16 | 59.2 | tRNA synthetases class I (M) |
XP_057767521.1 |
Pfam | PF01406 |
tRNA-synt_1e | Domain | 741 | 817 | 1.3e-08 | 35.3 | tRNA synthetases class I (C) catalytic domain |
XP_057767521.1 |
Pfam | PF08264 |
Anticodon_1 | Domain | 867 | 990 | 1.1e-14 | 55.3 | Anticodon-binding domain of tRNA ligase |
XP_057767521.1 |
Pfam | PF24810 |
RBD_LARS1 | Domain | 1016 | 1093 | 6.5e-08 | 33.2 | Leucine--tRNA ligase, RagD-binding domain |
XP_057767522.1 |
Pfam | PF00133 |
tRNA-synt_1 | Domain | 22 | 105 | 2.8e-29 | 102.8 | tRNA synthetases class I (I, L, M and V) |
XP_057767522.1 |
Pfam | PF09334 |
tRNA-synt_1g | Family | 52 | 111 | 4e-16 | 59.5 | tRNA synthetases class I (M) |
XP_057767522.1 |
Pfam | PF00133 |
tRNA-synt_1 | Domain | 198 | 758 | 2.8e-29 | 102.8 | tRNA synthetases class I (I, L, M and V) |
XP_057767522.1 |
Pfam | PF09334 |
tRNA-synt_1g | Family | 666 | 764 | 4e-16 | 59.5 | tRNA synthetases class I (M) |
XP_057767522.1 |
Pfam | PF01406 |
tRNA-synt_1e | Domain | 672 | 748 | 1.2e-08 | 35.4 | tRNA synthetases class I (C) catalytic domain |
XP_057767522.1 |
Pfam | PF08264 |
Anticodon_1 | Domain | 798 | 921 | 9.4e-15 | 55.5 | Anticodon-binding domain of tRNA ligase |
XP_057767522.1 |
Pfam | PF24810 |
RBD_LARS1 | Domain | 947 | 1024 | 6e-08 | 33.4 | Leucine--tRNA ligase, RagD-binding domain |
每个公开基因均提供参考注释对应的转录本序列；蛋白编码基因另提供 CDS 和蛋白序列。非编码 RNA 不适用 CDS 或蛋白下载。真实 accession 的版本后缀予以保留。
2 records
下载 cDNA FASTA2 records
下载 CDS FASTA2 records
下载 Protein FASTAupstream/downstream 最大各 10000 bp。负链基因会自动反向互补，使最终 FASTA 为转录方向 5′→3′。