Gene Card 基因详情

支持统一新 ID、NCBI Gene ID、GeneID、locus_tag、Transcript ID、Protein ID，以及已人工核对的文献基因名查询。页面会先解析到丹参统一基因，再整合坐标、序列、注释、亚细胞定位、EMS 突变和下载入口。

当前解析结果

`SmilChr06G000583`

染色体: Chr06 坐标: 4991629-5011157 链方向: - 类型: protein_coding

基本信息

统一新 Gene ID	`SmilChr06G000583`
名称	SmilChr06G000583
描述	dicer-like protein 4
染色体 / SeqID	Chr06 / NC_080392.1
坐标与链方向	4991629 - 5011157 / `-`

ID 对照

NCBI Gene ID	`LOC130988127`
GeneID	`130988127`
locus_tag	`-`
NCBI Name	`LOC130988127`
原始查询	`SmilChr06G000583`

查看完整 ID 对照表

功能注释

来源	GFF basic annotation; NCBI GO GAF; Pfam-A/HMMER; Arabidopsis/Rice homolog
描述	dicer-like protein 4
产物	dicer-like protein 4
Arabidopsis	`AT5G20320` (DCL4) dicer-like 4 [Source:NCBI gene (formerly Entrezgene);Acc:832154] identity=50.2; qcov=1.01; evalue=0; confidence=high
Rice	`Os04g0509300` (DCL4) - identity=47.8; qcov=1.01; evalue=0; confidence=high
GO	GO:0003723 (RNA binding); GO:0004525 (ribonuclease III activity); GO:0005515 (protein binding); GO:0005524 (ATP binding); GO:0005634 (nucleus); GO:0005737 (cytoplasm); GO:0030422 (siRNA processing)
KEGG	-
Pfam	PF00035 (dsrm); PF00270 (DEAD); PF00271 (Helicase_C); PF00636 (Ribonuclease_3); PF02170 (PAZ); PF03368 (Dicer_dimer); PF04851 (ResIII); PF14622 (Ribonucleas_3_3); PF14709 (DND1_DSRM)
InterPro	-
eggNOG	-
NR	GeneID:130988127

打开注释模块

亚细胞定位 Top 5

Rank	定位位置	中文	Score	Protein ID
1	Nucleus	细胞核	1.00000	`XP_057767884.1`
2	Cytoplasm	细胞质	0.40000	`XP_057767884.1`
3	Cytosol	细胞质基质	0.40000	`XP_057767884.1`
4	Chromosome	染色体	0.00000	`XP_057767884.1`
5	Centromere	着丝粒	0.00000	`XP_057767884.1`

进入亚细胞定位查询

EMS 诱变突变

下表显示该基因在丹参 EMS 材料中的前 12 个候选突变；完整结果可进入 EMS 突变库继续筛选或下载。

位置	REF	ALT	类型	影响	携带材料数	材料
`Chr6:4992297`	`G`	`A`	EMS_canonical_SNV	CDS_variant	2	B107,C24
`Chr6:4992312`	`G`	`A`	EMS_canonical_SNV	CDS_variant	1	C61
`Chr6:4992324`	`C`	`T`	EMS_canonical_SNV	CDS_variant	1	C43
`Chr6:4992584`	`C`	`T`	EMS_canonical_SNV	CDS_variant	1	C13
`Chr6:4992624`	`TTC`	`T`	2bp_deletion	frameshift_variant	1	C86
`Chr6:4992624`	`TTC`	`T`	2bp_deletion	CDS_variant	1	C86
`Chr6:4993262`	`C`	`T`	EMS_canonical_SNV	CDS_variant	1	D10
`Chr6:4993784`	`G`	`A`	EMS_canonical_SNV	CDS_variant	1	D10
`Chr6:4993881`	`C`	`T`	EMS_canonical_SNV	CDS_variant	5	B29,B52,C14,C44,C45
`Chr6:4993882`	`G`	`A`	EMS_canonical_SNV	CDS_variant	2	B23,C9
`Chr6:4994530`	`C`	`T`	EMS_canonical_SNV	CDS_variant	2	B68,C8
`Chr6:4994547`	`G`	`GTA`	2bp_insertion	frameshift_variant	8	B109,B115,B15,B20,B93,B99,C34,C51

进入 EMS 突变库

预测功能结构域

结构域结果来自 Pfam-A HMM 数据库和 HMMER hmmscan；位置为对应蛋白序列上的氨基酸坐标。

XP_057767884.1

1593 aa

796

1593

ResIII

DEAD

Helicase_C

Dicer_dimer

PAZ

Ribonuclease_3

Ribonucleas_3_3

Ribonuclease_3

DND1_DSRM

dsrm

PF04851 ResIII 44-210 aa PF00270 DEAD 46-209 aa PF00271 Helicase_C 382-497 aa PF03368 Dicer_dimer 564-648 aa PF02170 PAZ 870-972 aa PF00636 Ribonuclease_3 1017-1147 aa PF14622 Ribonucleas_3_3 1203-1333 aa PF00636 Ribonuclease_3 1222-1331 aa PF14709 DND1_DSRM 1512-1587 aa PF00035 dsrm 1517-1583 aa

Protein ID	来源	结构域编号	结构域名称	类型	起始 aa	终止 aa	E-value	Score	说明
`XP_057767884.1`	Pfam	`PF04851`	ResIII	Family	44	210	1.2e-16	61.8	Type III restriction enzyme, res subunit
`XP_057767884.1`	Pfam	`PF00270`	DEAD	Domain	46	209	1.9e-22	80.5	DEAD/DEAH box helicase
`XP_057767884.1`	Pfam	`PF00271`	Helicase_C	Domain	382	497	1e-19	71.5	Helicase conserved C-terminal domain
`XP_057767884.1`	Pfam	`PF03368`	Dicer_dimer	Domain	564	648	2.4e-26	92.3	Dicer dimerisation domain
`XP_057767884.1`	Pfam	`PF02170`	PAZ	Domain	870	972	3.2e-07	31.1	PAZ domain
`XP_057767884.1`	Pfam	`PF00636`	Ribonuclease_3	Family	1017	1147	3.7e-43	147.2	Ribonuclease III domain
`XP_057767884.1`	Pfam	`PF14622`	Ribonucleas_3_3	Family	1203	1333	1.2e-29	103.8	Ribonuclease-III-like
`XP_057767884.1`	Pfam	`PF00636`	Ribonuclease_3	Family	1222	1331	3.7e-43	147.2	Ribonuclease III domain
`XP_057767884.1`	Pfam	`PF14709`	DND1_DSRM	Domain	1512	1587	7.8e-15	55.7	double strand RNA binding domain from DEAD END PROTEIN 1
`XP_057767884.1`	Pfam	`PF00035`	dsrm	Domain	1517	1583	8.9e-15	55.7	Double-stranded RNA binding motif

cDNA / CDS / Protein 序列

下载基因组 DNA（仅基因区域）

每个公开基因均提供参考注释对应的转录本序列；蛋白编码基因另提供 CDS 和蛋白序列。非编码 RNA 不适用 CDS 或蛋白下载。真实 accession 的版本后缀予以保留。

cDNA

1 records

下载 cDNA FASTA

XM_057911901.1
TCATACACGTATCAGATTTCATGCTTTAAATGTAGCAGTAGGATTTCATAGTTTTAAATATGGTTGGACAGCACGTAGTGAGAATATATACAGATATGAACCACCTCCAACAAGCAAATATAGCAAACGAAAAGCCGCCATTTATTCCTCCGCTCTTCGTTGTTCTTGTCACATAACTCTGATTCCCTTCCCCCTCCATAAAGTGCAAAAAGAAAAGTCAGTTTCTTTTCTTACACAGCTACACCACCACCGTCAATTCA...

CDS

1 records

下载 CDS FASTA

LOC130988127
ATGGAGATCGGTGGTGAGAAAATTGATTTTGCGGAAAAAGAGGTTGTAGACCAACTTTCAGCTCTCTCTCTCTGCGGCGCTGAAGAGCAGAAAAAATCCCTGCAACAGGAGAAAGATCCGAGAAGAGTTGCTAGAAAGTATCAAACGGAGCTGTGTAAGAGAGCGTTGGAAGAGAACATCATCGTGTATTTGGGAACCGGTGCTGGAAAAACTCATATTGCTGTTTTACTTATGTATGAGATGGCTCACCTTTTCAAGAG...

Protein

1 records

下载 Protein FASTA

XP_057767884.1
MEIGGEKIDFAEKEVVDQLSALSLCGAEEQKKSLQQEKDPRRVARKYQTELCKRALEENIIVYLGTGAGKTHIAVLLMYEMAHLFKSPQKSVCIFLAPTTALIHQQAKVIEKSVDFKVGIYCGRTTRARSHEDWEKQIEEYEVLVMTPELMLHYLAHCFIKMEQIALLIFDECHHAQLDCDHQYAQIMKIFYKVDLVKCPRIFGMTASPKLGKGGSIDGLEAIMHSKVCTFEDENEVEQFVTSPKAIAYEYGSTEKSFLC...

上下游序列下载

upstream/downstream 最大各 10000 bp。负链基因会自动反向互补，使最终 FASTA 为转录方向 5′→3′。

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