Gene Card 基因详情

支持统一新 ID、NCBI Gene ID、GeneID、locus_tag、Transcript ID、Protein ID，以及已人工核对的文献基因名查询。页面会先解析到丹参统一基因，再整合坐标、序列、注释、亚细胞定位、EMS 突变和下载入口。

当前解析结果

`SmilChr07G000823`

染色体: Chr07 坐标: 5799258-5805833 链方向: + 类型: protein_coding

基本信息

统一新 Gene ID	`SmilChr07G000823`
名称	SmilChr07G000823
描述	methionine--tRNA ligase, chloroplastic/mitochondrial
染色体 / SeqID	Chr07 / NC_080393.1
坐标与链方向	5799258 - 5805833 / `+`

ID 对照

NCBI Gene ID	`LOC130991992`
GeneID	`130991992`
locus_tag	`-`
NCBI Name	`LOC130991992`
原始查询	`SmilChr07G000823`

查看完整 ID 对照表

功能注释

来源	GFF basic annotation; NCBI GO GAF; KEGG smil; Pfam-A/HMMER; Arabidopsis/Rice homolog
描述	methionine--tRNA ligase, chloroplastic/mitochondrial
产物	methionine--tRNA ligase%2C chloroplastic/mitochondrial
Arabidopsis	`AT3G55400` (OVA1) methionyl-tRNA synthetase / methionine-tRNA ligase / MetRS (cpMetRS) [Source:NCBI gene (formerly Entrezgene);Acc:824706] identity=71.1; qcov=1.03; evalue=0; confidence=high
Rice	`Os03g0209600` - identity=73.1; qcov=0.899; evalue=0; confidence=high
GO	GO:0004825 (methionine-tRNA ligase activity); GO:0005524 (ATP binding); GO:0005739 (mitochondrion); GO:0006431 (methionyl-tRNA aminoacylation); GO:0009570 (chloroplast stroma)
KEGG	smil00450 (Selenocompound metabolism - Salvia miltiorrhiza (redroot sage)); smil00970 (Aminoacyl-tRNA biosynthesis - Salvia miltiorrhiza (redroot sage)); smil01100 (Metabolic pathways - Salvia miltiorrhiza (redroot sage))
Pfam	PF00133 (tRNA-synt_1); PF01406 (tRNA-synt_1e); PF08264 (Anticodon_1); PF09334 (tRNA-synt_1g); PF19303 (Anticodon_3)
InterPro	-
eggNOG	-
NR	GeneID:130991992

打开注释模块

亚细胞定位 Top 5

Rank	定位位置	中文	Score	Protein ID
1	Chloroplast	叶绿体	1.00000	`XP_057772418.1`
2	Mitochondrion	线粒体	0.80000	`XP_057772418.1`
3	Nucleus	细胞核	0.00000	`XP_057772418.1`
4	Chromosome	染色体	0.00000	`XP_057772418.1`
5	Centromere	着丝粒	0.00000	`XP_057772418.1`

进入亚细胞定位查询

EMS 诱变突变

下表显示该基因在丹参 EMS 材料中的前 12 个候选突变；完整结果可进入 EMS 突变库继续筛选或下载。

位置	REF	ALT	类型	影响	携带材料数	材料
`Chr7:5799392`	`G`	`A`	EMS_canonical_SNV	CDS_variant	1	D10
`Chr7:5799490`	`G`	`A`	EMS_canonical_SNV	CDS_variant	2	B129,B40
`Chr7:5799514`	`C`	`T`	EMS_canonical_SNV	CDS_variant	1	A9
`Chr7:5799536`	`C`	`T`	EMS_canonical_SNV	CDS_variant	3	B12,B55,D7
`Chr7:5800025`	`C`	`T`	EMS_canonical_SNV	CDS_variant	5	A9,B12,B55,C9,D7
`Chr7:5800056`	`A`	`ACT`	2bp_insertion	frameshift_variant	14	B115,B14,B20,B24,B28,B34,B66,B67,B80,B94,C13,C34,C38,C56
`Chr7:5800485`	`C`	`T`	EMS_canonical_SNV	CDS_variant	1	B133
`Chr7:5802322`	`C`	`T`	EMS_canonical_SNV	CDS_variant	3	B115,B20,C8
`Chr7:5802620`	`C`	`T`	EMS_canonical_SNV	CDS_variant	1	B51
`Chr7:5803681`	`C`	`T`	EMS_canonical_SNV	CDS_variant	1	B67
`Chr7:5804289`	`C`	`T`	EMS_canonical_SNV	CDS_variant	4	A1,B33,B53,C77
`Chr7:5804323`	`G`	`A`	EMS_canonical_SNV	CDS_variant	1	C55

进入 EMS 突变库

预测功能结构域

结构域结果来自 Pfam-A HMM 数据库和 HMMER hmmscan；位置为对应蛋白序列上的氨基酸坐标。

XP_057772418.1

613 aa

306

613

tRNA-synt_1

tRNA-synt_1g

tRNA-synt_1e

tRNA-synt_1g

tRNA-synt_1

Anticodon_3

Anticodon_1

PF00133 tRNA-synt_1 54-109 aa PF09334 tRNA-synt_1g 59-197 aa PF01406 tRNA-synt_1e 70-225 aa PF09334 tRNA-synt_1g 193-417 aa PF00133 tRNA-synt_1 275-385 aa PF19303 Anticodon_3 434-570 aa PF08264 Anticodon_1 445-545 aa

XP_057772419.1

598 aa

299

598

tRNA-synt_1

tRNA-synt_1g

tRNA-synt_1e

tRNA-synt_1g

tRNA-synt_1

Anticodon_3

Anticodon_1

PF00133 tRNA-synt_1 54-109 aa PF09334 tRNA-synt_1g 59-197 aa PF01406 tRNA-synt_1e 70-225 aa PF09334 tRNA-synt_1g 192-417 aa PF00133 tRNA-synt_1 275-385 aa PF19303 Anticodon_3 434-570 aa PF08264 Anticodon_1 445-547 aa

XP_057772420.1

596 aa

298

596

tRNA-synt_1

tRNA-synt_1g

tRNA-synt_1e

tRNA-synt_1g

tRNA-synt_1

Anticodon_3

Anticodon_1

XP_057772421.1

596 aa

298

596

tRNA-synt_1

tRNA-synt_1g

tRNA-synt_1e

tRNA-synt_1g

tRNA-synt_1

Anticodon_3

Anticodon_1

Protein ID	来源	结构域编号	结构域名称	类型	起始 aa	终止 aa	E-value	Score	说明
`XP_057772418.1`	Pfam	`PF00133`	tRNA-synt_1	Domain	54	109	3.1e-25	89.5	tRNA synthetases class I (I, L, M and V)
`XP_057772418.1`	Pfam	`PF09334`	tRNA-synt_1g	Family	59	197	6.7e-107	358.4	tRNA synthetases class I (M)
`XP_057772418.1`	Pfam	`PF01406`	tRNA-synt_1e	Domain	70	225	4.4e-15	56.5	tRNA synthetases class I (C) catalytic domain
`XP_057772418.1`	Pfam	`PF09334`	tRNA-synt_1g	Family	193	417	6.7e-107	358.4	tRNA synthetases class I (M)
`XP_057772418.1`	Pfam	`PF00133`	tRNA-synt_1	Domain	275	385	3.1e-25	89.5	tRNA synthetases class I (I, L, M and V)
`XP_057772418.1`	Pfam	`PF19303`	Anticodon_3	Domain	434	570	1.8e-13	51.3	Anticodon binding domain of methionyl tRNA ligase
`XP_057772418.1`	Pfam	`PF08264`	Anticodon_1	Domain	445	545	2.6e-06	28.1	Anticodon-binding domain of tRNA ligase
`XP_057772419.1`	Pfam	`PF00133`	tRNA-synt_1	Domain	54	109	3.2e-25	89.5	tRNA synthetases class I (I, L, M and V)
`XP_057772419.1`	Pfam	`PF09334`	tRNA-synt_1g	Family	59	197	6e-107	358.5	tRNA synthetases class I (M)
`XP_057772419.1`	Pfam	`PF01406`	tRNA-synt_1e	Domain	70	225	4.2e-15	56.6	tRNA synthetases class I (C) catalytic domain
`XP_057772419.1`	Pfam	`PF09334`	tRNA-synt_1g	Family	192	417	6e-107	358.5	tRNA synthetases class I (M)
`XP_057772419.1`	Pfam	`PF00133`	tRNA-synt_1	Domain	275	385	3.2e-25	89.5	tRNA synthetases class I (I, L, M and V)
`XP_057772419.1`	Pfam	`PF19303`	Anticodon_3	Domain	434	570	1.7e-13	51.4	Anticodon binding domain of methionyl tRNA ligase
`XP_057772419.1`	Pfam	`PF08264`	Anticodon_1	Domain	445	547	2e-06	28.5	Anticodon-binding domain of tRNA ligase
`XP_057772420.1`	Pfam	`PF00133`	tRNA-synt_1	Domain	54	109	3.2e-25	89.5	tRNA synthetases class I (I, L, M and V)
`XP_057772420.1`	Pfam	`PF09334`	tRNA-synt_1g	Family	59	197	5.8e-107	358.6	tRNA synthetases class I (M)
`XP_057772420.1`	Pfam	`PF01406`	tRNA-synt_1e	Domain	70	225	4.2e-15	56.6	tRNA synthetases class I (C) catalytic domain
`XP_057772420.1`	Pfam	`PF09334`	tRNA-synt_1g	Family	192	417	5.8e-107	358.6	tRNA synthetases class I (M)
`XP_057772420.1`	Pfam	`PF00133`	tRNA-synt_1	Domain	275	385	3.2e-25	89.5	tRNA synthetases class I (I, L, M and V)
`XP_057772420.1`	Pfam	`PF19303`	Anticodon_3	Domain	434	570	1.7e-13	51.4	Anticodon binding domain of methionyl tRNA ligase
`XP_057772420.1`	Pfam	`PF08264`	Anticodon_1	Domain	445	545	2.5e-06	28.2	Anticodon-binding domain of tRNA ligase
`XP_057772421.1`	Pfam	`PF00133`	tRNA-synt_1	Domain	54	109	3.2e-25	89.5	tRNA synthetases class I (I, L, M and V)
`XP_057772421.1`	Pfam	`PF09334`	tRNA-synt_1g	Family	59	197	5.8e-107	358.6	tRNA synthetases class I (M)
`XP_057772421.1`	Pfam	`PF01406`	tRNA-synt_1e	Domain	70	225	4.2e-15	56.6	tRNA synthetases class I (C) catalytic domain
`XP_057772421.1`	Pfam	`PF09334`	tRNA-synt_1g	Family	192	417	5.8e-107	358.6	tRNA synthetases class I (M)
`XP_057772421.1`	Pfam	`PF00133`	tRNA-synt_1	Domain	275	385	3.2e-25	89.5	tRNA synthetases class I (I, L, M and V)
`XP_057772421.1`	Pfam	`PF19303`	Anticodon_3	Domain	434	570	1.7e-13	51.4	Anticodon binding domain of methionyl tRNA ligase
`XP_057772421.1`	Pfam	`PF08264`	Anticodon_1	Domain	445	545	2.5e-06	28.2	Anticodon-binding domain of tRNA ligase

cDNA / CDS / Protein 序列

下载基因组 DNA（仅基因区域）

每个公开基因均提供参考注释对应的转录本序列；蛋白编码基因另提供 CDS 和蛋白序列。非编码 RNA 不适用 CDS 或蛋白下载。真实 accession 的版本后缀予以保留。

cDNA

4 records

下载 cDNA FASTA

XM_057916436.1
AAAAATACTTACTCTCAACTTTTTTTTTTCTCTCTCTTTACAAAAATACCCTCTTTGCGTCATCTTCTCCACTCTCTCAGTGCTGCAGCCTCTCACACTCAACCAAACCACAATCTCTGGGCAGCATCAATGGCGTGTAGAGTTCAATACTCCATTCACAACAGCCTCCGCTTCTTCTCCTCCTCTCCATCGTCTCTCAATTTCTGCTCTTCTGCAAAACCACACAAAATTCGCTTCAGAAATTCTAAGTATGTCTCCAT...

XM_057916437.1
AAAATACCCTCTTTGCGTCATCTTCTCCACTCTCTCAGTGCTGCAGCCTCTCACACTCAACCAAACCACAATCTCTGGGCAGCATCAATGGCGTGTAGAGTTCAATACTCCATTCACAACAGCCTCCGCTTCTTCTCCTCCTCTCCATCGTCTCTCAATTTCTGCTCTTCTGCAAAACCACACAAAATTCGCTTCAGAAATTCTAAGTATGTCTCCATTTCTAAGGGCGCGCTGTACTGTACTTGCAGCGCAGACCCTTT...

CDS

4 records

下载 CDS FASTA

LOC130991992
ATGGCGTGTAGAGTTCAATACTCCATTCACAACAGCCTCCGCTTCTTCTCCTCCTCTCCATCGTCTCTCAATTTCTGCTCTTCTGCAAAACCACACAAAATTCGCTTCAGAAATTCTAAGTATGTCTCCATTTCTAAGGGCGCGCTGTACTGTACTTGCAGCGCAGACCCTTTCGTCCTCACCACTCCGCTTTATTACGTGAATGCCCCGCCCCATATGGGCAGCGCCTACACCACCATTGCCGCCGACGCCATCGCCCG...

Protein

4 records

下载 Protein FASTA

XP_057772418.1
MACRVQYSIHNSLRFFSSSPSSLNFCSSAKPHKIRFRNSKYVSISKGALYCTCSADPFVLTTPLYYVNAPPHMGSAYTTIAADAIARFQRLLGKKVIFITGTDEHGEKIASAAMANGSSPSKHCDSVSQAYRALWRELDISNDKFIRTTNPNHEAIVKEFYSRVLANGDIYRADYEGLYCVNCEEYKDEKELLENNCCPVHLKPCLDRKEDNYFFALSKYQKQLEQILEENPDFVQPSYRLHEVQGWVKRGLRDFSISRA...

XP_057772419.1
MACRVQYSIHNSLRFFSSSPSSLNFCSSAKPHKIRFRNSKYVSISKGALYCTCSADPFVLTTPLYYVNAPPHMGSAYTTIAADAIARFQRLLGKKVIFITGTDEHGEKIASAAMANGSSPSKHCDSVSQAYRALWRELDISNDKFIRTTNPNHEAIVKEFYSRVLANGDIYRADYEGLYCVNCEEYKDEKELLENNCCPVHLKPCLDRKEDNYFFALSKYQKQLEQILEENPDFVQPSYRLHEVQGWVKRGLRDFSISRA...

上下游序列下载

upstream/downstream 最大各 10000 bp。负链基因会自动反向互补，使最终 FASTA 为转录方向 5′→3′。

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