Gene Card 基因详情

支持统一新 ID、NCBI Gene ID、GeneID、locus_tag、Transcript ID、Protein ID，以及已人工核对的文献基因名查询。页面会先解析到丹参统一基因，再整合坐标、序列、注释、亚细胞定位、EMS 突变和下载入口。

当前解析结果

`SmilChr08G000042`

染色体: Chr08 坐标: 275874-279015 链方向: - 类型: protein_coding

基本信息

统一新 Gene ID	`SmilChr08G000042`
名称	SmilChr08G000042
描述	vacuolar protein sorting-associated protein 20 homolog 2
染色体 / SeqID	Chr08 / NC_080394.1
坐标与链方向	275874 - 279015 / `-`

ID 对照

NCBI Gene ID	`LOC130999668`
GeneID	`130999668`
locus_tag	`-`
NCBI Name	`LOC130999668`
原始查询	`SmilChr08G000042`

查看完整 ID 对照表

功能注释

来源	GFF basic annotation; NCBI GO GAF; KEGG smil; Pfam-A/HMMER; Arabidopsis/Rice homolog
描述	vacuolar protein sorting-associated protein 20 homolog 2
产物	vacuolar protein sorting-associated protein 20 homolog 2
Arabidopsis	`AT5G09260` (VPS20.2) vacuolar protein sorting-associated protein 20.2 [Source:NCBI gene (formerly Entrezgene);Acc:830785] identity=74.9; qcov=1; evalue=1.7e-115; confidence=high
Rice	`Os02g0596500` - identity=63.6; qcov=1.06; evalue=2.19e-98; confidence=high
GO	GO:0000815 (ESCRT III complex); GO:0005771 (multivesicular body); GO:0006900 (vesicle budding from membrane); GO:0032511 (late endosome to vacuole transport via multivesicular body sorting pathway)
KEGG	smil03250 (Viral life cycle - HIV-1 - Salvia miltiorrhiza (redroot sage)); smil04144 (Endocytosis - Salvia miltiorrhiza (redroot sage))
Pfam	PF03357 (Snf7)
InterPro	-
eggNOG	-
NR	GeneID:130999668

打开注释模块

亚细胞定位 Top 5

Rank	定位位置	中文	Score	Protein ID
1	Cytosol	细胞质基质	1.00000	`XP_057781255.1`
2	Cytoplasmic vesicle	细胞质囊泡	1.00000	`XP_057781256.1`
3	Golgi apparatus	高尔基体	1.00000	`XP_057781256.1`
4	Trans-golgi network	Trans-golgi network	1.00000	`XP_057781256.1`
5	Vesicle	囊泡	1.00000	`XP_057781256.1`

进入亚细胞定位查询

EMS 诱变突变

下表显示该基因在丹参 EMS 材料中的前 12 个候选突变；完整结果可进入 EMS 突变库继续筛选或下载。

位置	REF	ALT	类型	影响	携带材料数	材料
`Chr8:276202`	`G`	`GT`	1bp_insertion	frameshift_variant	15	A4,A5,B18,B48,B53,B55,B86,B88,C12,C2,C22,C28,C33,C6,C75
`Chr8:276282`	`AGG`	`A`	2bp_deletion	frameshift_variant	56	A11,A8,B101,B102,B109,B115,B117,B122,B125,B127,B131,B132,B139,B140,B18,B20,B28,B33,B41,B42,B43,B52,B54,B60,B69,B70,B77,B79,B80,B82,B83,B86,B87,B89,B90,B92,B93,C2,C20,C25,C27,C35,C4,C40,C41,C42,C48,C53,C55,C62,C63,C75,C82,C85,D11,D2
`Chr8:276285`	`A`	`ATG`	2bp_insertion	frameshift_variant	56	A11,A8,B101,B102,B109,B115,B117,B122,B125,B127,B131,B132,B139,B140,B18,B20,B28,B33,B41,B42,B43,B52,B54,B60,B69,B70,B77,B79,B80,B82,B83,B86,B87,B89,B90,B92,B93,C2,C20,C25,C27,C35,C4,C40,C41,C42,C48,C53,C55,C62,C63,C75,C82,C85,D11,D2
`Chr8:276292`	`G`	`A`	EMS_canonical_SNV	CDS_variant	2	B131,D11
`Chr8:276342`	`C`	`T`	EMS_canonical_SNV	CDS_variant	3	B102,C69,D6
`Chr8:276369`	`C`	`T`	EMS_canonical_SNV	CDS_variant	1	B115
`Chr8:276730`	`C`	`T`	EMS_canonical_SNV	CDS_variant	1	C78
`Chr8:276762`	`C`	`T`	EMS_canonical_SNV	CDS_variant	4	B38,B39,B71,C62
`Chr8:277488`	`C`	`T`	EMS_canonical_SNV	CDS_variant	2	A1,C84
`Chr8:277509`	`G`	`A`	EMS_canonical_SNV	CDS_variant	4	C50,C78,D11,D4
`Chr8:277533`	`C`	`T`	EMS_canonical_SNV	CDS_variant	2	A21,D2

进入 EMS 突变库

预测功能结构域

结构域结果来自 Pfam-A HMM 数据库和 HMMER hmmscan；位置为对应蛋白序列上的氨基酸坐标。

XP_057781255.1

234 aa

117

234

Snf7

PF03357 Snf7 17-195 aa

XP_057781256.1

223 aa

112

223

Snf7

PF03357 Snf7 17-195 aa

Protein ID	来源	结构域编号	结构域名称	类型	起始 aa	终止 aa	E-value	Score	说明
`XP_057781255.1`	Pfam	`PF03357`	Snf7	Family	17	195	2.4e-40	138.7	Snf7
`XP_057781256.1`	Pfam	`PF03357`	Snf7	Family	17	195	1.9e-40	139.0	Snf7

cDNA / CDS / Protein 序列

下载基因组 DNA（仅基因区域）

每个公开基因均提供参考注释对应的转录本序列；蛋白编码基因另提供 CDS 和蛋白序列。非编码 RNA 不适用 CDS 或蛋白下载。真实 accession 的版本后缀予以保留。

cDNA

2 records

下载 cDNA FASTA

XM_057925273.1
AAAAGAACATTGCCTCGTCGATTTCATCTTCGTTGTTGTCCAATTCCACAGTCTGTCTCTCTCTCGATCTCGATGACATTTGAGCTGAGAGAGATATCTCTGCTGTGGATTTGATGCGCAAGCCGCATGATATTTCACCTGTCAATTTTTTGAAGTTTTCTGAAAGGGATTGAGCCGAAAAAATGGGGAATGTTTTTGTGAAGAGACCGAAGATCACGGAGGTGGACAGAGCCATTCTTTCCCTCAAAACTCAACGGCGG...

XM_057925272.1
TTGCCTCGTCGATTTCATCTTCGTTGTTGTCCAATTCCACAGTCTGTCTCTCTCTCGATCTCGATGACATTTGAGCTGAGAGAGATATCTCTGCTGTGGATTTGATGCGCAAGCCGCATGATATTTCACCTGTCAATTTTTTGAAGTTTTCTGAAAGGGATTGAGCCGAAAAAATGGGGAATGTTTTTGTGAAGAGACCGAAGATCACGGAGGTGGACAGAGCCATTCTTTCCCTCAAAACTCAACGGCGGAAGCTCGGT...

CDS

2 records

下载 CDS FASTA

LOC130999668
ATGGGGAATGTTTTTGTGAAGAGACCGAAGATCACGGAGGTGGACAGAGCCATTCTTTCCCTCAAAACTCAACGGCGGAAGCTCGGTCAATACCAGCAGCAGCTAGAGGCTGTCATTGATGCTGAAAAGCAAGCTGCAAAAGATTTACTTCGGGAAAAGAAGAAAGACAGGGCATTGTTAGCATTGAAAAAGAAGAAGGTTCAAGAAGACCTGTTAAAGCAAGTTGACGGTTGGCTTATCAATGTAGAGCAGCAATTAGC...

Protein

2 records

下载 Protein FASTA

XP_057781255.1
MGNVFVKRPKITEVDRAILSLKTQRRKLGQYQQQLEAVIDAEKQAAKDLLREKKKDRALLALKKKKVQEDLLKQVDGWLINVEQQLADVELASKQKAVFESLKSGNNAVKAMQSEISLDDVQKLMDDSAEAKAYQEDLNAILGEKLSAEDEEEVLAEFDNLEAQLTLGDLPKVPSAAPSTQEPEDELDLPDVPTKAPAMSEAVASSSSEISEHRKGLSLSLSLSPPPPPPPHHC

XP_057781256.1
MGNVFVKRPKITEVDRAILSLKTQRRKLGQYQQQLEAVIDAEKQAAKDLLREKKKDRALLALKKKKVQEDLLKQVDGWLINVEQQLADVELASKQKAVFESLKSGNNAVKAMQSEISLDDVQKLMDDSAEAKAYQEDLNAILGEKLSAEDEEEVLAEFDNLEAQLTLGDLPKVPSAAPSTQEPEDELDLPDVPTKAPAMSEAVASSSSEISEHRKVMEEPLAA

上下游序列下载

upstream/downstream 最大各 10000 bp。负链基因会自动反向互补，使最终 FASTA 为转录方向 5′→3′。

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