Gene Card 基因详情

支持统一新 ID、NCBI Gene ID、GeneID、locus_tag、Transcript ID、Protein ID，以及已人工核对的文献基因名查询。页面会先解析到丹参统一基因，再整合坐标、序列、注释、亚细胞定位、EMS 突变和下载入口。

当前解析结果

`SmilChr08G000187`

染色体: Chr08 坐标: 1263710-1266485 链方向: - 类型: protein_coding

基本信息

统一新 Gene ID	`SmilChr08G000187`
名称	SmilChr08G000187
描述	malate dehydrogenase
染色体 / SeqID	Chr08 / NC_080394.1
坐标与链方向	1263710 - 1266485 / `-`

ID 对照

NCBI Gene ID	`LOC130999789`
GeneID	`130999789`
locus_tag	`-`
NCBI Name	`LOC130999789`
原始查询	`SmilChr08G000187`

查看完整 ID 对照表

功能注释

来源	GFF basic annotation; NCBI GO GAF; KEGG smil; Pfam-A/HMMER; Arabidopsis/Rice homolog
描述	malate dehydrogenase
产物	malate dehydrogenase
Arabidopsis	`AT5G43330` (c-NAD-MDH2) Lactate/malate dehydrogenase family protein [Source:NCBI gene (formerly Entrezgene);Acc:834351] identity=91; qcov=1; evalue=0; confidence=high
Rice	`Os10g0478200` (OscMDH) - identity=89.8; qcov=1; evalue=0; confidence=high
GO	GO:0006099 (tricarboxylic acid cycle); GO:0006107 (oxaloacetate metabolic process); GO:0006108 (malate metabolic process); GO:0006734 (GO:0006734); GO:0030060 (L-malate dehydrogenase (NAD+) activity)
KEGG	smil00020 (Citrate cycle (TCA cycle) - Salvia miltiorrhiza (redroot sage)); smil00270 (Cysteine and methionine metabolism - Salvia miltiorrhiza (redroot sage)); smil00566 (Sulfoquinovose metabolism - Salvia miltiorrhiza (redroot sage)); smil00620 (Pyruvate metabolism - Salvia miltiorrhiza (redroot sage)); smil00630 (Glyoxylate and dicarboxylate metabolism - Salvia miltiorrhiza (redroot sage)); smil00710 (Carbon fixation by Calvin cycle - Salvia miltiorrhiza (redroot sage)); smil01100 (Metabolic pathways - Salvia miltiorrhiza (redroot sage)); smil01110 (Biosynthesis of secondary metabolites - Salvia miltiorrhiza (redroot sage)); smil01200 (Carbon metabolism - Salvia miltiorrhiza (redroot sage))
Pfam	PF00056 (Ldh_1_N); PF02866 (Ldh_1_C)
InterPro	-
eggNOG	-
NR	GeneID:130999789

打开注释模块

亚细胞定位 Top 5

Rank	定位位置	中文	Score	Protein ID
1	Cytoplasm	细胞质	1.00000	`XP_057781410.1`
2	Cytosol	细胞质基质	1.00000	`XP_057781410.1`
3	Nucleoplasm	核质	0.20000	`XP_057781411.1`
4	Nucleus	细胞核	0.00000	`XP_057781410.1`
5	Chromosome	染色体	0.00000	`XP_057781410.1`

进入亚细胞定位查询

EMS 诱变突变

下表显示该基因在丹参 EMS 材料中的前 12 个候选突变；完整结果可进入 EMS 突变库继续筛选或下载。

位置	REF	ALT	类型	影响	携带材料数	材料
`Chr8:1264044`	`G`	`A`	EMS_canonical_SNV	stop_gained	12	B112,B5,B79,B83,B87,C11,C12,C20,C69,C8,C85,D3
`Chr8:1264074`	`G`	`A`	EMS_canonical_SNV	stop_gained	74	A1,A13,A2,A20,A24,A6,A7,B100,B107,B108,B111,B117,B120,B121,B125,B129,B13,B130,B131,B135,B136,B137,B17,B2,B26,B27,B3,B33,B34,B35,B45,B46,B48,B50,B55,B62,B69,B71,B74,B8,B86,B89,B90,B91,B92,B93,B98,C12,C13,C14,C16,C23,C3,C33,C36,C38,C40,C44,C46,C51,C56,C62,C64,C66,C72,C74,C75,C76,C81,C82,C84,D11,D4,D8
`Chr8:1264222`	`G`	`A`	EMS_canonical_SNV	CDS_variant	4	B34,B50,C14,D11
`Chr8:1264342`	`G`	`A`	EMS_canonical_SNV	CDS_variant	1	C25
`Chr8:1264727`	`C`	`T`	EMS_canonical_SNV	CDS_variant	1	B84
`Chr8:1265195`	`G`	`A`	EMS_canonical_SNV	CDS_variant	1	B78
`Chr8:1265479`	`C`	`T`	EMS_canonical_SNV	CDS_variant	4	B111,B123,B25,C1
`Chr8:1265507`	`C`	`T`	EMS_canonical_SNV	CDS_variant	3	B126,B57,C79

进入 EMS 突变库

预测功能结构域

结构域结果来自 Pfam-A HMM 数据库和 HMMER hmmscan；位置为对应蛋白序列上的氨基酸坐标。

XP_057781410.1

333 aa

166

333

Ldh_1_N

Ldh_1_C

PF00056 Ldh_1_N 6-153 aa PF02866 Ldh_1_C 157-306 aa

XP_057781411.1

332 aa

166

332

Ldh_1_N

Ldh_1_C

PF00056 Ldh_1_N 6-153 aa PF02866 Ldh_1_C 157-326 aa

Protein ID	来源	结构域编号	结构域名称	类型	起始 aa	终止 aa	E-value	Score	说明
`XP_057781410.1`	Pfam	`PF00056`	Ldh_1_N	Domain	6	153	1.1e-31	110.4	lactate/malate dehydrogenase, NAD binding domain
`XP_057781410.1`	Pfam	`PF02866`	Ldh_1_C	Domain	157	306	5.2e-39	134.7	lactate/malate dehydrogenase, alpha/beta C-terminal domain
`XP_057781411.1`	Pfam	`PF00056`	Ldh_1_N	Domain	6	153	1.1e-31	110.5	lactate/malate dehydrogenase, NAD binding domain
`XP_057781411.1`	Pfam	`PF02866`	Ldh_1_C	Domain	157	326	7.1e-45	153.8	lactate/malate dehydrogenase, alpha/beta C-terminal domain

cDNA / CDS / Protein 序列

下载基因组 DNA（仅基因区域）

每个公开基因均提供参考注释对应的转录本序列；蛋白编码基因另提供 CDS 和蛋白序列。非编码 RNA 不适用 CDS 或蛋白下载。真实 accession 的版本后缀予以保留。

cDNA

2 records

下载 cDNA FASTA

XM_057925428.1
TTTGACATAAGTCCACCTCACAAGTCACAAACCACGAGTCGCGTGGTAACGACGTCGTATTGACTTCTTCCTTTGGTTTTGTTCGAAATTTTCTACTGATAAATATCCTCACACTCTTTCACTCTTCATCTCTCGTTTTCTCCTCTCTCGAAACTCTCAAAAAGCTATCTTCGTTTCCATCGGATTTTTTTCAGATCGCAGATCTAATGGCCAAGGATCCAGTCCGTGTTCTCGTCACCGGCGCTGCTGGGCAAATTGGA...

XM_057925427.1
CCACGAGTCGCGTGGTAACGACGTCGTATTGACTTCTTCCTTTGGTTTTGTTCGAAATTTTCTACTGATAAATATCCTCACACTCTTTCACTCTTCATCTCTCGTTTTCTCCTCTCTCGAAACTCTCAAAAAGCTATCTTCGTTTCCATCGGATTTTTTTCAGATCGCAGATCTAATGGCCAAGGATCCAGTCCGTGTTCTCGTCACCGGCGCTGCTGGGCAAATTGGATATGCTCTTGTTCCGATGATTGCTAGGGGAG...

CDS

2 records

下载 CDS FASTA

LOC130999789
ATGGCCAAGGATCCAGTCCGTGTTCTCGTCACCGGCGCTGCTGGGCAAATTGGATATGCTCTTGTTCCGATGATTGCTAGGGGAGTTATGTTGGGCGCGGACCAGCCTGTGATCCTCCACATGTTGGATATTCCACCTGCTGCGGAGGCACTTAATGGTGTTAAGATGGAGTTGGTGGATGCAGCATTTCCACTTCTTAAAGGTGTTGTTGCCACCACTGATGCCGTTGAAGCTTGCACTGGTGTCAATATTGCTGTGAT...

Protein

2 records

下载 Protein FASTA

XP_057781410.1
MAKDPVRVLVTGAAGQIGYALVPMIARGVMLGADQPVILHMLDIPPAAEALNGVKMELVDAAFPLLKGVVATTDAVEACTGVNIAVMVGGFPRKEGMERKDVMSKNVSIYKSQASALEKHAAANCKVLVVANPANTNALILKEFAPSIPEKNITCLTRLDHNRALGQISERLNVQVSDVKNVIIWGNHSSTQYPDVTHATVNTPAGEKPVRELVADDKWLNEEFIAVVQQRGAAIIKARKLSSALSAASSACDHIRDWVL...

XP_057781411.1
MAKDPVRVLVTGAAGQIGYALVPMIARGVMLGADQPVILHMLDIPPAAEALNGVKMELVDAAFPLLKGVVATTDAVEACTGVNIAVMVGGFPRKEGMERKDVMSKNVSIYKSQASALEKHAAANCKVLVVANPANTNALILKEFAPSIPEKNITCLTRLDHNRALGQISERLNVQVSDVKNVIIWGNHSSTQYPDVTHATVNTPAGEKPVRELVADDKWLNEEFIAVVQQRGAAIIKARKLSSALSAASSACDHIRDWVL...

上下游序列下载

upstream/downstream 最大各 10000 bp。负链基因会自动反向互补，使最终 FASTA 为转录方向 5′→3′。

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