Gene Card 基因详情

支持统一新 ID、NCBI Gene ID、GeneID、locus_tag、Transcript ID、Protein ID，以及已人工核对的文献基因名查询。页面会先解析到丹参统一基因，再整合坐标、序列、注释、亚细胞定位、EMS 突变和下载入口。

当前解析结果

`SmilChr08G000443`

染色体: Chr08 坐标: 3163850-3166811 链方向: - 类型: protein_coding

基本信息

统一新 Gene ID	`SmilChr08G000443`
名称	SmilChr08G000443
描述	E3 ubiquitin-protein ligase RSL1-like
染色体 / SeqID	Chr08 / NC_080394.1
坐标与链方向	3163850 - 3166811 / `-`

ID 对照

NCBI Gene ID	`LOC131000025`
GeneID	`131000025`
locus_tag	`-`
NCBI Name	`LOC131000025`
原始查询	`SmilChr08G000443`

查看完整 ID 对照表

功能注释

来源	GFF basic annotation; NCBI GO GAF; Pfam-A/HMMER; Arabidopsis/Rice homolog
描述	E3 ubiquitin-protein ligase RSL1-like
产物	E3 ubiquitin-protein ligase RSL1-like
Arabidopsis	`AT5G60250` (AT5G60250) zinc finger (C3HC4-type RING finger) family protein [Source:NCBI gene (formerly Entrezgene);Acc:836147] identity=36.1; qcov=1.09; evalue=2.13e-103; confidence=medium
Rice	`Os12g0631200` - identity=30.8; qcov=0.781; evalue=2.5e-46; confidence=low
GO	GO:0000151 (ubiquitin ligase complex); GO:0000209 (protein polyubiquitination); GO:0003676 (nucleic acid binding); GO:0004523 (RNA-DNA hybrid ribonuclease activity); GO:0005737 (cytoplasm); GO:0006511 (ubiquitin-dependent protein catabolic process); GO:0008270 (zinc ion binding); GO:0031624 (ubiquitin conjugating enzyme binding); GO:0032436 (positive regulation of proteasomal ubiquitin-dependent protein catabolic process); GO:0061630 (ubiquitin protein ligase activity)
KEGG	-
Pfam	PF00097 (zf-C3HC4); PF01485 (IBR); PF13456 (RVT_3); PF22191 (IBR_1)
InterPro	-
eggNOG	-
NR	GeneID:131000025

打开注释模块

亚细胞定位 Top 5

Rank	定位位置	中文	Score	Protein ID
1	Nucleus	细胞核	1.00000	`XP_057781743.1`
2	Cytoplasm	细胞质	0.60000	`XP_057781743.1`
3	Cytosol	细胞质基质	0.40000	`XP_057781743.1`
4	Chromosome	染色体	0.00000	`XP_057781743.1`
5	Centromere	着丝粒	0.00000	`XP_057781743.1`

进入亚细胞定位查询

EMS 诱变突变

下表显示该基因在丹参 EMS 材料中的前 12 个候选突变；完整结果可进入 EMS 突变库继续筛选或下载。

位置	REF	ALT	类型	影响	携带材料数	材料
`Chr8:3164115`	`G`	`A`	EMS_canonical_SNV	stop_gained	1	B117
`Chr8:3164115`	`G`	`A`	EMS_canonical_SNV	CDS_variant	1	B117
`Chr8:3164131`	`G`	`A`	EMS_canonical_SNV	CDS_variant	2	B93,C41
`Chr8:3164182`	`G`	`A`	EMS_canonical_SNV	CDS_variant	3	B44,C26,C34
`Chr8:3164922`	`G`	`A`	EMS_canonical_SNV	CDS_variant	1	B59
`Chr8:3165007`	`G`	`A`	EMS_canonical_SNV	CDS_variant	5	B69,C13,C34,C50,C75
`Chr8:3165027`	`C`	`T`	EMS_canonical_SNV	CDS_variant	2	B5,B58
`Chr8:3165063`	`C`	`T`	EMS_canonical_SNV	CDS_variant	3	B56,B96,D7
`Chr8:3165111`	`G`	`A`	EMS_canonical_SNV	CDS_variant	1	B32
`Chr8:3165155`	`C`	`T`	EMS_canonical_SNV	CDS_variant	3	A23,B38,B39
`Chr8:3165190`	`C`	`T`	EMS_canonical_SNV	CDS_variant	4	B56,B9,B96,D7
`Chr8:3165213`	`C`	`T`	EMS_canonical_SNV	CDS_variant	3	A18,B3,B81

进入 EMS 突变库

预测功能结构域

结构域结果来自 Pfam-A HMM 数据库和 HMMER hmmscan；位置为对应蛋白序列上的氨基酸坐标。

XP_057781743.1

479 aa

240

479

RVT_3

zf-C3HC4

IBR

IBR_1

PF13456 RVT_3 122-233 aa PF00097 zf-C3HC4 259-303 aa PF01485 IBR 339-391 aa PF01485 IBR 406-459 aa PF22191 IBR_1 419-456 aa

Protein ID	来源	结构域编号	结构域名称	类型	起始 aa	终止 aa	E-value	Score	说明
`XP_057781743.1`	Pfam	`PF13456`	RVT_3	Domain	122	233	2.2e-11	44.4	Reverse transcriptase-like
`XP_057781743.1`	Pfam	`PF00097`	zf-C3HC4	Domain	259	303	2.7e-05	24.7	Zinc finger, C3HC4 type (RING finger)
`XP_057781743.1`	Pfam	`PF01485`	IBR	Domain	339	391	6.1e-15	55.7	IBR domain, a half RING-finger domain
`XP_057781743.1`	Pfam	`PF01485`	IBR	Domain	406	459	6.1e-15	55.7	IBR domain, a half RING-finger domain
`XP_057781743.1`	Pfam	`PF22191`	IBR_1	Domain	419	456	3.4e-06	27.5	IBR domain

cDNA / CDS / Protein 序列

下载基因组 DNA（仅基因区域）

每个公开基因均提供参考注释对应的转录本序列；蛋白编码基因另提供 CDS 和蛋白序列。非编码 RNA 不适用 CDS 或蛋白下载。真实 accession 的版本后缀予以保留。

cDNA

1 records

下载 cDNA FASTA

XM_057925760.1
AGAAAAAAAAAAGAATAAAAGAAAGCAGCGGAGAAGCAGTGAGAGAGTTGACAAGCTGCGACTTCGACTCTTTCGCGCGAAGCCATTGAAGAAAGCAATAATGGCGGACGACTCCGAGATGGCCTTCAACCTCCAACTGGAAGAAGCCATTGCCGCCTCCCTAATCGACGGCGCCGCCGCGCCGCCCCCCGAAACCGACGGCGGAGGCCCCTATGACGCCCTCGTCGGGTGCGAACAGGAATTCCTCTACGACGTAATGG...

CDS

1 records

下载 CDS FASTA

LOC131000025
ATGGCGGACGACTCCGAGATGGCCTTCAACCTCCAACTGGAAGAAGCCATTGCCGCCTCCCTAATCGACGGCGCCGCCGCGCCGCCCCCCGAAACCGACGGCGGAGGCCCCTATGACGCCCTCGTCGGGTGCGAACAGGAATTCCTCTACGACGTAATGGCCGAGGTGGAGGCGGAGCGCCTGCGTCTCGACCTCCTCCGCCAGGTCCACGACCGCGCCCTCGCCTGCGAGATCATGAGCGTGCCGGACGAGGAGTGGAT...

Protein

1 records

下载 Protein FASTA

XP_057781743.1
MADDSEMAFNLQLEEAIAASLIDGAAAPPPETDGGGPYDALVGCEQEFLYDVMAEVEAERLRLDLLRQVHDRALACEIMSVPDEEWIKTGDNLHRPFGEGSSSSSNGENFEFKVYVKGLVEGEASGIGAAICDGNDGLLFELSKGLSGKDQQTNKELVDFKALVEGLDSAVMLDLKRVTIVTDNPLLHQHITGENTQTIKSAVALSNQIHLLLRKFTLVHASLVASNDNKSVKLARNAIAFQVNRSAGSSNRKNQTECCT...

上下游序列下载

upstream/downstream 最大各 10000 bp。负链基因会自动反向互补，使最终 FASTA 为转录方向 5′→3′。

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JBrowse

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