当前解析结果
SmilChr08G000810| 统一新 Gene ID | SmilChr08G000810 |
|---|---|
| 名称 | SmilChr08G000810 |
| 描述 | probable pre-mRNA-splicing factor ATP-dependent RNA helicase DEAH2 |
| 染色体 / SeqID | Chr08 / NC_080394.1 |
| 坐标与链方向 | 5965133 - 5969214 / + |
| NCBI Gene ID | LOC131000392 |
|---|---|
| GeneID | 131000392 |
| locus_tag | - |
| NCBI Name | LOC131000392 |
| 原始查询 | SmilChr08G000810 |
| 来源 | GFF basic annotation; NCBI GO GAF; KEGG smil; Pfam-A/HMMER; Arabidopsis/Rice homolog |
|---|---|
| 描述 | probable pre-mRNA-splicing factor ATP-dependent RNA helicase DEAH2 |
| 产物 | probable pre-mRNA-splicing factor ATP-dependent RNA helicase DEAH2 |
| Arabidopsis | AT3G62310 (AT3G62310)RNA helicase family protein [Source:NCBI gene (formerly Entrezgene);Acc:825404] identity=85.1; qcov=1; evalue=0; confidence=high |
| Rice | Os03g0314100 (PRP-22)- identity=83.3; qcov=0.996; evalue=0; confidence=high |
| GO | GO:0003723 (RNA binding); GO:0003724 (RNA helicase activity); GO:0005524 (ATP binding); GO:0005622 (intracellular anatomical structure) |
| KEGG | smil03040 (Spliceosome - Salvia miltiorrhiza (redroot sage)) |
| Pfam | PF00270 (DEAD); PF00271 (Helicase_C); PF04408 (WHD_HA2); PF07717 (OB_NTP_bind); PF13245 (AAA_19); PF13401 (AAA_22); PF21010 (HA2_C) |
| InterPro | - |
| eggNOG | - |
| NR | GeneID:131000392 |
| Rank | 定位位置 | 中文 | Score | Protein ID |
|---|---|---|---|---|
| 1 | Nucleus | 细胞核 | 1.00000 | XP_057782248.1 |
| 2 | Cytosol | 细胞质基质 | 0.80000 | XP_057782249.1 |
| 3 | Cytoplasm | 细胞质 | 0.80000 | XP_057782250.1 |
| 4 | Nucleoplasm | 核质 | 0.40000 | XP_057782250.1 |
| 5 | Chromosome | 染色体 | 0.00000 | XP_057782249.1 |
下表显示该基因在丹参 EMS 材料中的前 12 个候选突变；完整结果可进入 EMS 突变库继续筛选或下载。
| 位置 | REF | ALT | 类型 | 影响 | 携带材料数 | 材料 |
|---|---|---|---|---|---|---|
Chr8:5965466 |
C |
T |
EMS_canonical_SNV | CDS_variant | 1 | B88 |
Chr8:5965491 |
C |
T |
EMS_canonical_SNV | CDS_variant | 3 | B35,B74,C25 |
Chr8:5965559 |
C |
T |
EMS_canonical_SNV | CDS_variant | 1 | B88 |
Chr8:5966184 |
GCT |
G |
2bp_deletion | frameshift_variant | 27 | A15,A20,A9,B105,B127,B137,B14,B24,B32,B51,B71,B78,B97,B99,C19,C25,C27,C31,C32,C34,C35,C45,C46,C48,C64,C66,C74 |
Chr8:5966188 |
T |
TGC |
2bp_insertion | frameshift_variant | 28 | A15,A20,A9,B105,B127,B137,B14,B16,B24,B32,B51,B71,B78,B97,B99,C19,C25,C27,C31,C32,C34,C35,C45,C46,C48,C64,C66,C74 |
Chr8:5966332 |
G |
A |
EMS_canonical_SNV | CDS_variant | 2 | C6,C80 |
Chr8:5966520 |
C |
T |
EMS_canonical_SNV | CDS_variant | 1 | B88 |
Chr8:5966526 |
C |
T |
EMS_canonical_SNV | CDS_variant | 1 | B98 |
Chr8:5966619 |
C |
T |
EMS_canonical_SNV | CDS_variant | 3 | B35,B74,C25 |
Chr8:5966769 |
C |
T |
EMS_canonical_SNV | CDS_variant | 1 | D11 |
Chr8:5966849 |
C |
T |
EMS_canonical_SNV | CDS_variant | 2 | B131,B82 |
Chr8:5966850 |
G |
A |
EMS_canonical_SNV | CDS_variant | 1 | B88 |
结构域结果来自 Pfam-A HMM 数据库和 HMMER hmmscan；位置为对应蛋白序列上的氨基酸坐标。
XP_057782248.1PF00270
DEAD 71-226 aa
PF13401
AAA_22 81-220 aa
PF00271
Helicase_C 266-406 aa
PF04408
WHD_HA2 469-497 aa
PF21010
HA2_C 498-557 aa
PF07717
OB_NTP_bind 619-695 aa
XP_057782249.1PF00270
DEAD 71-226 aa
PF13245
AAA_19 78-210 aa
PF13401
AAA_22 81-220 aa
PF00271
Helicase_C 266-406 aa
PF04408
WHD_HA2 469-497 aa
PF21010
HA2_C 498-527 aa
XP_057782250.1PF00270
DEAD 71-226 aa
PF13245
AAA_19 78-210 aa
PF13401
AAA_22 81-220 aa
PF00271
Helicase_C 266-406 aa
PF04408
WHD_HA2 469-497 aa
PF21010
HA2_C 498-526 aa
| Protein ID | 来源 | 结构域编号 | 结构域名称 | 类型 | 起始 aa | 终止 aa | E-value | Score | 说明 |
|---|---|---|---|---|---|---|---|---|---|
XP_057782248.1 |
Pfam | PF00270 |
DEAD | Domain | 71 | 226 | 8.9e-09 | 36.0 | DEAD/DEAH box helicase |
XP_057782248.1 |
Pfam | PF13401 |
AAA_22 | Domain | 81 | 220 | 4.7e-07 | 30.9 | AAA domain |
XP_057782248.1 |
Pfam | PF00271 |
Helicase_C | Domain | 266 | 406 | 5.3e-10 | 40.2 | Helicase conserved C-terminal domain |
XP_057782248.1 |
Pfam | PF04408 |
WHD_HA2 | Domain | 469 | 497 | 1.3e-11 | 44.9 | Helicase associated domain (HA2), winged-helix |
XP_057782248.1 |
Pfam | PF21010 |
HA2_C | Domain | 498 | 557 | 1.9e-15 | 57.8 | Helicase associated domain (HA2), ratchet-like |
XP_057782248.1 |
Pfam | PF07717 |
OB_NTP_bind | Domain | 619 | 695 | 4e-25 | 88.5 | Oligonucleotide/oligosaccharide-binding (OB)-fold |
XP_057782249.1 |
Pfam | PF00270 |
DEAD | Domain | 71 | 226 | 6e-09 | 36.6 | DEAD/DEAH box helicase |
XP_057782249.1 |
Pfam | PF13245 |
AAA_19 | Domain | 78 | 210 | 9.9e-06 | 26.5 | AAA domain |
XP_057782249.1 |
Pfam | PF13401 |
AAA_22 | Domain | 81 | 220 | 3.3e-07 | 31.4 | AAA domain |
XP_057782249.1 |
Pfam | PF00271 |
Helicase_C | Domain | 266 | 406 | 3.6e-10 | 40.8 | Helicase conserved C-terminal domain |
XP_057782249.1 |
Pfam | PF04408 |
WHD_HA2 | Domain | 469 | 497 | 9.7e-12 | 45.3 | Helicase associated domain (HA2), winged-helix |
XP_057782249.1 |
Pfam | PF21010 |
HA2_C | Domain | 498 | 527 | 9.2e-08 | 33.2 | Helicase associated domain (HA2), ratchet-like |
XP_057782250.1 |
Pfam | PF00270 |
DEAD | Domain | 71 | 226 | 5.7e-09 | 36.6 | DEAD/DEAH box helicase |
XP_057782250.1 |
Pfam | PF13245 |
AAA_19 | Domain | 78 | 210 | 9.8e-06 | 26.5 | AAA domain |
XP_057782250.1 |
Pfam | PF13401 |
AAA_22 | Domain | 81 | 220 | 3e-07 | 31.5 | AAA domain |
XP_057782250.1 |
Pfam | PF00271 |
Helicase_C | Domain | 266 | 406 | 3.4e-10 | 40.8 | Helicase conserved C-terminal domain |
XP_057782250.1 |
Pfam | PF04408 |
WHD_HA2 | Domain | 469 | 497 | 9.3e-12 | 45.4 | Helicase associated domain (HA2), winged-helix |
XP_057782250.1 |
Pfam | PF21010 |
HA2_C | Domain | 498 | 526 | 3.7e-07 | 31.3 | Helicase associated domain (HA2), ratchet-like |
每个公开基因均提供参考注释对应的转录本序列；蛋白编码基因另提供 CDS 和蛋白序列。非编码 RNA 不适用 CDS 或蛋白下载。真实 accession 的版本后缀予以保留。
3 records
下载 cDNA FASTA3 records
下载 CDS FASTA3 records
下载 Protein FASTAupstream/downstream 最大各 10000 bp。负链基因会自动反向互补，使最终 FASTA 为转录方向 5′→3′。